SpecificityThis antibody has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins. Typically less than 1% cross-reactivity against other types of apolipoprotein was detected by ELISA against purified standards. Non-specific cross-reaction of anti-apolipoprotein antibodies with other Human serum proteins is negligible.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
FunctionApolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Involvement in diseaseHypobetalipoproteinemia, familial, 1 Familial ligand-defective apolipoprotein B-100 Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apolipoprotein B antibody (ab98132)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human liver tissue labelled with ab98132 at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein B antibody (ab98132)This image is courtesy of an anonymous Abreview
ab98132 staining Apolipoprotein B in Mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (0.5 ug/ml in BSA/TBS buffer) for 30 minutes. ab97101 Rabbit polyclonal Secondary Antibody to Anti-goat HRP, IgG - H&L, pre-adsorbed (1/500) was used as the secondary antibody.