Recombinant
RabMAb

Anti-Apolipoprotein B antibody [EPR2914] (ab139401)

Overview

  • Product name
    Anti-Apolipoprotein B antibody [EPR2914]
    See all Apolipoprotein B primary antibodies
  • Description
    Rabbit monoclonal [EPR2914] to Apolipoprotein B
  • Tested applications
    Suitable for: Flow Cyt, IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Human plasma and Human serum lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab139401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Predicted molecular weight: 515 kDa.Can be blocked with Human Apolipoprotein B peptide (ab199783).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
    • Involvement in disease
      Hypobetalipoproteinemia, familial, 1
      Familial ligand-defective apolipoprotein B-100
      Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
    • Sequence similarities
      Contains 1 vitellogenin domain.
    • Post-translational
      modifications
      Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
    • Cellular localization
      Cytoplasm. Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apo B-100 antibody
      • Apo B-48 antibody
      • ApoB 100 antibody
      • ApoB 48 antibody
      • APOB antibody
      • APOB_HUMAN antibody
      • Apolipoprotein B (including Ag(x) antigen) antibody
      • Apolipoprotein B 100 antibody
      • Apolipoprotein B antibody
      • Apolipoprotein B-48 antibody
      • Apolipoprotein B48 antibody
      • FLDB antibody
      • LDLCQ4 antibody
      see all

    Images

    • Overlay histogram showing HepG2 cells stained with ab139401 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139401, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Apolipoprotein B antibody [EPR2914] (ab139401) at 1/10000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human serum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 515 kDa
    • Western blot analysis on Immunoprecipitation pellet from human serum lysate using anti-Apolipoprotein B RabMAb (ab139401).

    References

    ab139401 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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