FITC Anti-Apolipoprotein B antibody (ab27637)
Key features and details
- FITC Goat polyclonal to Apolipoprotein B
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-Apolipoprotein B antibody
See all Apolipoprotein B primary antibodies -
Description
FITC Goat polyclonal to Apolipoprotein B -
Host species
Goat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
This antibody is specific for human apo B-100 (apolipoprotein B). -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native LDL protein (purified)
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General notes
Molar F/P ratio is 6.1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 0.2% PBS, 0.0146% EDTA, 0.435% Sodium chloride, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified by human apo B-100-Sepharose™ affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. -
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia. -
Sequence similarities
Contains 1 vitellogenin domain. -
Post-translational
modificationsPalmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
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Database links
- Entrez Gene: 338 Human
- Omim: 107730 Human
- SwissProt: P04114 Human
- Unigene: 120759 Human
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Alternative names
- Apo B 100 antibody
- Apo B antibody
- Apo B-100 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab27637 has been referenced in 3 publications.
- Chetty VK et al. Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35565197
- Zhou J et al. High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations. Sci Adv 6:N/A (2020). PubMed: 33219024
- Pang B et al. Quality Assessment and Comparison of Plasma-Derived Extracellular Vesicles Separated by Three Commercial Kits for Prostate Cancer Diagnosis. Int J Nanomedicine 15:10241-10256 (2020). PubMed: 33364756