Apolipoprotein B (APOB) Human ELISA kit (ab108807)

Overview

  • Product nameApolipoprotein B (APOB) Human ELISA kit
    See all Apolipoprotein B kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.4%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell culture extracts, Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.008 µg/ml
  • Range
    0.008 µg/ml - 0.25 µg/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Apolipoprotein B (APOB) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein B concentrations in Human plasma, serum, CSF and cell culture samples.

    An Apolipoprotein B specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein B specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein B captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Cellular localizationCell Membrane, Cytoplasmic and Secreted
  • Alternative names
    • Apo B
    • Apo B 100
    • Apo B-100
    • Apo B-100
    • Apo B-48
    • Apo B-48
    • ApoB
    • APOB
    • ApoB 100
    • ApoB 48
    • APOB protein
    • APOB_HUMAN
    • Apolipoprotein B
    • apolipoprotein B (including Ag(x) antigen)
    • Apolipoprotein B 100
    • Apolipoprotein B 48
    • Apolipoprotein B-100
    • Apolipoprotein B-48
    • Apolipoprotein B-48
    • Apolipoprotein B100
    • FLDB
    • LDLCQ4
    • mutant Apo B 100
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Apolipoprotein B (APOB) Human ELISA kit images

  • Apolipoprotein B measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108807

Protocols

References for Apolipoprotein B (APOB) Human ELISA kit (ab108807)

ab108807 has not yet been referenced specifically in any publications.

Product Wall

Merci pour votre émail.

Nous n'avons pas besoin de plus de formalités. Je vais mettre en l'envoi du nouveau kit.

Nous sommes désolés que l'ancien kit que vous avez reçu ne fonctionne pas comme attendu. Voici le numéro de commande ...

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Only the assays for APOB (ab108807) and APOE (ab108813) have been tested with CSF. They are suitable for this kind of sample. The APOA1 assays have not been tested with CSF yet, but there are plans to test ab108803. Kit ab108804, the competitive ELISA ...

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Me temo que el kit ab108807 no puede distinguir entre las isoformas Apo B48 y Apo B100.

The plate is Corning Costar® 2592. It should fit into most plate washers. The wash buffer contains 0.1% Tween -20.



Q1: Will this kit detect mouse ApoB?
A1: No.

Q2: Is it known whether this kit will detect ApoB48 or ApoB100? Or both?
A2: It has not been determined if this kit will specifically detect ApoB48 or ApoB100.

Thank you for your inquiry and I apologize for the delay in this reply.

The lyophilized powder standard is supplied at 0.75 ug. Reconstitute the 0.75 ug of Apo B Standard with 3 ml of EIA Diluent to generate a solution of 0.25 ug/ml. Any left...

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Hello. The cross reactivity was deternmined by purified protein and plasma samples combination. It is not by single determinants. Please let me know if you have any further questions.



I believe this kit includes 0.75ug of standard. Based on the protocol, this provides you 3 mls of 0.25ug/ml of the highest concentration of standard. This starting concentration is subsequently diluted in 2x serial dilutions to produce the cu...

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Thank you for contacting us. There is no cross-reactivity with mouse for ab108807. Please let me know if I can be of further assistance.


Here is the shelf life information you requested:
ab108804 - 6 months if kit is unused and reagents stored at 4 deg C or -20 deg C as appropriate; 1 month after opening and limiting reagent is microtiter plate storage
ab108807 - 6 months...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"