The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Conjugation: Use at an assay dependent dilution.
ELISA: 1/2500 - 1/20000.
WB: 1/2500 - 1/20000. Predicted molecular weight: 9 kDa. Dilution optimised using Chromogenic detection.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Appears to modulate the interaction of APOE with beta-migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification.
Synthesized mainly in liver and to a minor degree in intestine. Secreted in plasma.