Anti-Apolipoprotein CII antibody (ab7618)

Overview

  • Product name
    Anti-Apolipoprotein CII antibody
    See all Apolipoprotein CII primary antibodies
  • Description
    Goat polyclonal to Apolipoprotein CII
  • Specificity
    Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein C-II and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-III, E and J apoLipoproteins.
  • Tested applications
    Suitable for: Dot blot, ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native apoLipoprotein Type C-II (purified).

  • General notes
    This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.


    At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.

Properties

Applications

Our Abpromise guarantee covers the use of ab7618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/5000 - 1/10000.
ELISA 1/4000 - 1/8000.
IHC-P 1/50 - 1/200.
WB Use at an assay dependent dilution.

Target

  • Relevance
    At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein C-II (apoCII) is in found in chylomicrons (large lipoprotein particles absorbed from the gastrointestinal tract) and VLDL (large lipoproteins that are broken down to eventually form LDL). ApoCII activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • APC 2 antibody
    • APC2 antibody
    • Apo CII antibody
    • APOC 2 antibody
    • ApoC II antibody
    • APOC2 antibody
    • APOC2 protein antibody
    • APOC2_HUMAN antibody
    • ApoCII antibody
    • Apolipoprotein C II antibody
    • Apolipoprotein C II precursor antibody
    • Apolipoprotein C2 antibody
    • ApolipoproteinCII antibody
    • MGC75082 antibody
    see all

References

ab7618 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry and for the detailed protocol. We are very sorry to hear that your customer is having problem with this antibody. We have searched our database and found that this is a popular selling product and your feedback is the fir...

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No I'm afraid that we do not have such a protocol - the protocols section of our website features suggested protocols for standard techniques. We are unable to suggest methods for use with specialised equipment.

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