Anti-Apolipoprotein CIII antibody (ab21032)
Key features and details
- Rabbit polyclonal to Apolipoprotein CIII
- Suitable for: ICC/IF, Sandwich ELISA, ELISA, WB
- Reacts with: Human, Monkey
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Apolipoprotein CIII antibody
See all Apolipoprotein CIII primary antibodies -
Description
Rabbit polyclonal to Apolipoprotein CIII -
Host species
Rabbit -
Specificity
Specifically binds to human Apo CIII. -
Tested applications
Suitable for: ICC/IF, Sandwich ELISA, ELISA, WBmore details -
Species reactivity
Reacts with: Human, Monkey -
Immunogen
Full length protein corresponding to Human Apolipoprotein CIII. Native protein
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.0292% EDTA, 0.435% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified by Human Apo CIII-Sepharose affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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Sandwich ELISA |
Use at an assay dependent concentration. PubMed: 21371998
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ELISA |
1/5000 - 1/40000. Can be paired for ELISA with Biotin Goat polyclonal to Apolipoprotein CIII (ab21024).
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
Sandwich ELISA
Use at an assay dependent concentration. PubMed: 21371998 |
ELISA
1/5000 - 1/40000. Can be paired for ELISA with Biotin Goat polyclonal to Apolipoprotein CIII (ab21024). |
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. |
Target
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Function
Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. -
Tissue specificity
Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine. -
Involvement in disease
Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). -
Sequence similarities
Belongs to the apolipoprotein C3 family. -
Post-translational
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 345 Human
- Omim: 107720 Human
- SwissProt: P02656 Human
- Unigene: 73849 Human
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Alternative names
- APOC3 antibody
- APO C3 antibody
- Apo CIII antibody
see all
Images
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All lanes : Anti-Apolipoprotein CIII antibody (ab21032) at 1 µg/ml
Lane 1 : Native Human Apolipoprotein CIII (ab77944) at 0.1 µg
Lane 2 : Native Human Apolipoprotein CIII (ab77944) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Exposure time: 1 minute -
ICC/IF image of ab21032 stained HepG2 (Human liver hepatocellular carcinoma cell line) cells.
The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21032, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa-Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa-Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour.
DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab21032 has been referenced in 5 publications.
- Yang M et al. Alantolactone suppresses APOC3 expression and alters lipid homeostasis in L02 liver cells. Eur J Pharmacol 828:60-66 (2018). PubMed: 29555504
- Khetarpal SA et al. A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels. Nat Med 23:1086-1094 (2017). PubMed: 28825717
- Schjoldager KT et al. Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc finger nuclease glycoengineered SimpleCells. Proc Natl Acad Sci U S A 109:9893-8 (2012). IP ; Human . PubMed: 22566642
- Swarup V et al. Identification and quantification of differentially expressed proteins in plasma of spinocerebellar ataxia type 12. Neurosci Res 73:161-7 (2012). PubMed: 22426495
- Wang Y et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). Sandwich ELISA ; Cynomolgus monkey . PubMed: 21371998