Overview

  • Product nameAnti-Apolipoprotein CIII antibody
    See all Apolipoprotein CIII primary antibodies
  • Description
    Rabbit polyclonal to Apolipoprotein CIII
  • SpecificitySpecifically binds to human Apo CIII.
  • Tested applicationsSuitable for: Sandwich ELISA, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Full length protein corresponding to Human Apolipoprotein CIII .

Properties

Applications

Our Abpromise guarantee covers the use of ab21032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. PubMed: 21371998
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/5000 - 1/40000. Can be paired for ELISA with Goat polyclonal to Apolipoprotein CIII (Biotin) (ab21024).
WB Use at an assay dependent dilution. Predicted molecular weight: 11 kDa.

Target

  • FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • Involvement in diseaseDefects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
  • Sequence similaritiesBelongs to the apolipoprotein C3 family.
  • Post-translational
    modifications
    O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • APOC3 antibody
    • APO C3 antibody
    • Apo CIII antibody
    • Apo-CIII antibody
    • APOC 3 antibody
    • ApoC III antibody
    • ApoC-III antibody
    • APOC3 antibody
    • APOC3_HUMAN antibody
    • ApoCIII antibody
    • Apolipoprotein C III antibody
    • Apolipoprotein C-III antibody
    • Apolipoprotein C3 antibody
    • ApolipoproteinCIII antibody
    • MGC150353 antibody
    see all

Anti-Apolipoprotein CIII antibody images

  • ICC/IF image of ab21032 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21032, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Apolipoprotein CIII antibody (ab21032) at 1 µg/ml

    Lane 1 : Natural Human Apolipoprotein CIII (ab77944) at 0.1 µg
    Lane 2 : Natural Human Apolipoprotein CIII (ab77944) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute
  • Sandwich ELISA, detecting Apolipoprotein CIII levels in Human or cynomolgus monkey serum, using ab21032 as capture antibody.

    Anti-human Apolipoprotein CIII polyclonal antibody (ab21032 at 10 µg/ml) was added to an ELISA plate and incubated overnight at 4°C. Blocking buffer was added and incubated for 1 hour at room temperature. 50 µl of serum or standard was added to each well and incubated for 2 hours at room temperature. After washing, 50 µl/well of detection antibody (ab21024) was added and incubated at room temperature 1 hour followed by 50 µl/well of Streptavidin-HRP (ab64269). After color development, the absorbance was read at 450nm.

References for Anti-Apolipoprotein CIII antibody (ab21032)

This product has been referenced in:
  • Schjoldager KT  et al. Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc finger nuclease glycoengineered SimpleCells. Proc Natl Acad Sci U S A 109:9893-8 (2012). IP ; Human . Read more (PubMed: 22566642) »
  • Swarup V  et al. Identification and quantification of differentially expressed proteins in plasma of spinocerebellar ataxia type 12. Neurosci Res 73:161-7 (2012). Read more (PubMed: 22426495) »

See all 3 Publications for this product

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