ICC/IF image of ab21032 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21032, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Apolipoprotein CIII antibody (ab21032)
All lanes : Anti-Apolipoprotein CIII antibody (ab21032) at 1 µg/ml
Lane 1 :Natural Human Apolipoprotein CIII (ab77944) at 0.1 µg Lane 2 :Natural Human Apolipoprotein CIII (ab77944) at 0.01 µg
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Exposure time: 1 minute
Sandwich ELISA - Anti-Apolipoprotein CIII antibody (ab21032)Image from Wang Y et al., J Lipid Res. 2011 Jun;52(6):1265-71. Epub 2011 Mar 2. Fig 2.; doi: 10.1194/jlr.D011148; June 2011, The Journal of Lipid Research, 52, 1265-1271.
Sandwich ELISA, detecting Apolipoprotein CIII levels in Human or cynomolgus monkey serum, using ab21032 as capture antibody.
Anti-human Apolipoprotein CIII polyclonal antibody (ab21032 at 10 µg/ml) was added to an ELISA plate and incubated overnight at 4°C. Blocking buffer was added and incubated for 1 hour at room temperature. 50 µl of serum or standard was added to each well and incubated for 2 hours at room temperature. After washing, 50 µl/well of detection antibody (ab21024) was added and incubated at room temperature 1 hour followed by 50 µl/well of Streptavidin-HRP (ab64269). After color development, the absorbance was read at 450nm.
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