Overview

  • Product nameAnti-Apolipoprotein CIII antibody (Biotin)
    See all Apolipoprotein CIII primary antibodies
  • Description
    Goat polyclonal to Apolipoprotein CIII (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Purified human Apolipoprotein CIII.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab21024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. PubMed: 21371998
WB 1/8000 - 1/30000. Predicted molecular weight: 11 kDa. Dilution optimised using Chromogenic detection.
ELISA 1/8000 - 1/30000. Can be paired for ELISA with Rabbit polyclonal to Apolipoprotein CIII (ab21032).

Target

  • FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • Involvement in diseaseDefects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
  • Sequence similaritiesBelongs to the apolipoprotein C3 family.
  • Post-translational
    modifications
    O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • APOC3 antibody
    • APO C3 antibody
    • Apo CIII antibody
    • Apo-CIII antibody
    • APOC 3 antibody
    • ApoC III antibody
    • ApoC-III antibody
    • APOC3 antibody
    • APOC3_HUMAN antibody
    • ApoCIII antibody
    • Apolipoprotein C III antibody
    • Apolipoprotein C-III antibody
    • Apolipoprotein C3 antibody
    • ApolipoproteinCIII antibody
    • MGC150353 antibody
    see all

Anti-Apolipoprotein CIII antibody (Biotin) images

  • Sandwich ELISA, detecting Apolipoprotein CIII levels in Human or cynomolgus monkey serum, using ab21024 as detection antibody.

    Anti-human Apolipoprotein CIII polyclonal antibody (ab21032) was added to an ELISA plate and incubated overnight at 4°C. Blocking buffer was added and incubated for 1 hour at room temperature. 50 µl of serum or standard was added to each well and incubated for 2 hours at room temperature. After washing, 50 µl/well of detection antibody (ab21024 at 1 µg/ml) was added and incubated at room temperature 1 hour followed by 50 µl/well of Streptavidin-HRP (ab64269). After color development, the absorbance was read at 450nm.

References for Anti-Apolipoprotein CIII antibody (Biotin) (ab21024)

This product has been referenced in:
  • Wang Y  et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). Sandwich ELISA ; Cynomolgus Monkey . Read more (PubMed: 21371998) »
  • Dernick G  et al. Multidimensional profiling of plasma lipoproteins by size exclusion chromatography followed by reverse-phase protein arrays. J Lipid Res 52:2323-31 (2011). Read more (PubMed: 21971713) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"