Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)
Key features and details
- Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
-
Product name
Anti-Apolipoprotein CIII antibody [EP1372Y]
See all Apolipoprotein CIII primary antibodies -
Description
Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Human liver tissue and plasma lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1372Y -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
|
Notes |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
Target
-
Function
Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. -
Tissue specificity
Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine. -
Involvement in disease
Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). -
Sequence similarities
Belongs to the apolipoprotein C3 family. -
Post-translational
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 345 Human
- Omim: 107720 Human
- SwissProt: P02656 Human
- Unigene: 73849 Human
-
Alternative names
- APOC3 antibody
- APO C3 antibody
- Apo CIII antibody
see all
Images
-
Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305) at 1/10000 dilution + Human plasma lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Please note this image was obtained using a purified batch. The current WB image of the Human liver is obtained using an unpurified batch.
-
Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305) at 1/2000 dilution + Human liver tissue lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab76305 has been referenced in 4 publications.
- Li L et al. Proteomics analysis of potential serum biomarkers for insulin resistance in patients with polycystic ovary syndrome. Int J Mol Med 45:1409-1416 (2020). PubMed: 32323743
- Lv P et al. Apolipoprotein C-III in the high-density lipoprotein proteome of cerebral lacunar infarction patients impairs its anti-inflammatory function. Int J Mol Med 41:61-68 (2018). PubMed: 29115584
- Zhang H et al. Characterization of apolipoprotein C1 in hepatitis C virus infection and morphogenesis. Virology 524:1-9 (2018). PubMed: 30130702
- Wang Y et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). PubMed: 21371998