For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide corresponding to the C terminus of human Apolipoprotein CIII.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab76305 in the following tested applications.
|WB||1/2000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||Use at an assay dependent concentration.|
ab76305 staining Apolipoprotein CIII in mouse liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 3% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/500 in0.3M PBST with 10% donkey serum) at 4°C for 24 hours. An Alexa Fluor® 488-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein CIII with ab76305 at 1/1000. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated monoclonal donkey antibody at 1/3000 dilution was used as the secondary antibody. Nuclei were counterstained with Hoechst at high power magnification.
ab76305 has not yet been referenced specifically in any publications.