Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)


  • Product nameAnti-Apolipoprotein CIII antibody [EP1372Y]
    See all Apolipoprotein CIII primary antibodies
  • Description
    Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII
  • Tested applicationsSuitable for: ICC/IF, WB, IP, Flow Cyt, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to the C terminus of human Apolipoprotein CIII.

  • Positive control
    • Human liver tissue lysate, Rat primary astrocytes.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab76305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/2000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP 1/30.
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration.


  • FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • Involvement in diseaseDefects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
  • Sequence similaritiesBelongs to the apolipoprotein C3 family.
  • Post-translational
    O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • APOC3 antibody
    • APO C3 antibody
    • Apo CIII antibody
    • Apo-CIII antibody
    • APOC 3 antibody
    • ApoC III antibody
    • ApoC-III antibody
    • APOC3 antibody
    • APOC3_HUMAN antibody
    • ApoCIII antibody
    • Apolipoprotein C III antibody
    • Apolipoprotein C-III antibody
    • Apolipoprotein C3 antibody
    • ApolipoproteinCIII antibody
    • MGC150353 antibody
    see all

Anti-Apolipoprotein CIII antibody [EP1372Y] images

  • Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305) at 1/2000 dilution + Human liver tissue lysate at 10 µg

    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
  • ab76305 staining Apolipoprotein CIII in mouse liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 3% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/500 in0.3M PBST with 10% donkey serum) at 4°C for 24 hours. An Alexa Fluor® 488-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.

  • Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein CIII with ab76305 at 1/1000. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated monoclonal donkey antibody at 1/3000 dilution was used as the secondary antibody. Nuclei were counterstained with Hoechst at high power magnification.

    See Abreview

References for Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)

ab76305 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary astrocytes culture)
Permeabilization Yes - 0.1M PBS with 1% Triton X
Specification Primary astrocytes culture
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Methanol

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 06 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Liver)
Permeabilization Yes - 0.1 M PBS with 3% Triton X
Specification Liver
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 05 2016

Thank you for contacting us.

Please find attached the WB protocol;

Please find attached the link which actually explains the reason of band at ˜70-80 kDa. T...

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Application Western blot
Sample Human Cell lysate - whole cell (BREAST (MCF-7))
Loading amount 0.2 µg
Specification BREAST (MCF-7)
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 01 2010

Application Flow Cytometry
Sample Human Cell (U266 cell line B lymphocyte (myeloma))
Specification U266 cell line B lymphocyte (myeloma)
Preparation Cell harvesting/tissue preparation method: suspension cells, so used directly
Sample buffer: RPMI 1640 medium with 2 mM L-glutamine
Fixation Unfixed
Permeabilization No
Gating Strategy total stained cells

Prof. Paul Townsend

Verified customer

Submitted May 07 2010