Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)

Overview

  • Product nameAnti-Apolipoprotein CIII antibody [EP1372Y]
    See all Apolipoprotein CIII primary antibodies
  • Description
    Rabbit monoclonal [EP1372Y] to Apolipoprotein CIII
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to the C terminus of human Apolipoprotein CIII.

  • Positive control
    • Human liver tissue lysate, Rat primary astrocytes.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP 1/30.

Target

  • FunctionInhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles.
  • Tissue specificityConstitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine.
  • Involvement in diseaseDefects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
  • Sequence similaritiesBelongs to the apolipoprotein C3 family.
  • Post-translational
    modifications
    O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • APOC3 antibody
    • APO C3 antibody
    • Apo CIII antibody
    • Apo-CIII antibody
    • APOC 3 antibody
    • ApoC III antibody
    • ApoC-III antibody
    • APOC3 antibody
    • APOC3_HUMAN antibody
    • ApoCIII antibody
    • Apolipoprotein C III antibody
    • Apolipoprotein C-III antibody
    • Apolipoprotein C3 antibody
    • ApolipoproteinCIII antibody
    • MGC150353 antibody
    see all

Anti-Apolipoprotein CIII antibody [EP1372Y] images

  • Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305) at 1/2000 dilution + Human liver tissue lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa

References for Anti-Apolipoprotein CIII antibody [EP1372Y] (ab76305)

ab76305 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary astrocytes culture)
Permeabilization Yes - 0.1M PBS with 1% Triton X
Specification Primary astrocytes culture
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Methanol
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 06 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Liver)
Permeabilization Yes - 0.1 M PBS with 3% Triton X
Specification Liver
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 05 2016

Thank you for contacting us.

Please find attached the WB protocol;

http://www.abcam.com/index.html?pageconfig=resource&rid=13045

Please find attached the link which actually explains the reason of band at ˜70-80 kDa. T...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (BREAST (MCF-7))
Loading amount 0.2 µg
Specification BREAST (MCF-7)
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 01 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (U266 cell line B lymphocyte (myeloma))
Permeabilization No
Gating Strategy total stained cells
Specification U266 cell line B lymphocyte (myeloma)
Preparation Cell harvesting/tissue preparation method: suspension cells, so used directly
Sample buffer: RPMI 1640 medium with 2 mM L-glutamine
Fixation Unfixed
Username

Prof. Paul Townsend

Verified customer

Submitted May 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"