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Recombinant full length protein produced in E. coli: FHLGKCPNPP VQENFDVNKY PGRWYEIEKI PTTFENGRCI QANYSLMENG KIKVLNQELR ADGTVNQIEG EATPVNLTEP AKLEVKFSWF MPSAPYHILA TDYENYALVY SCTSISQSFH VDFAWILARN VALPPETVDS LKNILTSNNI DVKKMTVTDQ VNCPKLSAHH HHHH, corresponding to amino acids 23-189 of Human Apolipoprotein D. Contains a C terminal His-tag of 7 extra AA. The amino acid sequence corresponds to the UniProt P05090. The following modifications were made: Four amino acid exchanges were introduced at the surface of ApoD (Trp99His, Cys116Ser, Ile118Ser, Leu120Ser) to enhance the solubility of the recombinant protein and another three (Leu23Pro, Pro133Val, Asn134Ala) to facilitate its genetic manipulation.
Our Abpromise guarantee covers the use of ab113634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.|
|ELISA||Use at an assay dependent dilution.|
ab113634 has not yet been referenced specifically in any publications.