Anti-Apo-D antibody (ab191275)
Key features and details
- Goat polyclonal to Apo-D
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Apo-D antibody
See all Apo-D primary antibodies -
Description
Goat polyclonal to Apo-D -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human Apo-D aa 1-100 (internal sequence).
Database link: P05090 -
Positive control
- Formalin-Fixed, Paraffin-Embedded Human Kidney & adrenal tissue, Human liver lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa).
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa). |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. -
Tissue specificity
Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 100968527 Chimpanzee
- Entrez Gene: 101146024 Gorilla
- Entrez Gene: 347 Human
- Omim: 107740 Human
- SwissProt: P05090 Human
- Unigene: 522555 Human
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Alternative names
- APO D antibody
- Apo-D antibody
- ApoD antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Apo-D with ab191275 at 5 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal tissue labeling Apo-D with ab191275 at 5 µg/ml.
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Anti-Apo-D antibody (ab191275) at 0.3 µg/ml + Human liver lysate in RIPA buffer at 35 µg
Predicted band size: 21 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab191275 has not yet been referenced specifically in any publications.