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Full length purified Mouse Apolipoprotein E from pooled mouse plasma high and very low density lipoproteins.
Our Abpromise guarantee covers the use of ab20874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|RID||Use at an assay dependent concentration.|
|Immunodiffusion||Use at an assay dependent concentration.|
|Immunoelectrophoresis||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Predicted molecular weight: 36 kDa. Dilution optimised using Chromogenic detection.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20505763|
This image is courtesy of S Kanazir, Inst Biol Res, Yugoslavia
Rabbit polyclonal to Apolipoprotein E (ab20874) in western blot analysis of proteins isolated from 100mg of rat brain hippocampaus. Tissue was homogenized in a 5V of gentle lysis buffer (10mM HEPES, 1mM EGTA, 5mM MgCl2, 142,5mM KCl, 0,25% NP-40, with Roche complete Protease Inhibitor Cocktail Tablets) and centrifuged 20 mins/14000rpm/4oC. Supernatants reserved and protein concentrations were assigned by Bradford method. Proteins were resolved onto 10% SDS-PAGE and blotted on nitrocellulose membranes (Amersham- Hybond C). The membranes were incubated with anti-ApoE (ab20874 at 1/400) for 2h at rm temp in 0.05% TBST (150 mM NaCl, 50 mM Tris, pH 7.0, and 0.05% Tween 20) with 3% milk. Following incubation with the anti-rabbit HRP-labeled secondary antibody (1/10000 in 0.05% TBST, 1h rm temp), blots were developed using the enhanced chemiluminescent substrate (ECL, Amersham Bioscience).
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