Overview

  • Product nameAnti-Apolipoprotein F antibody
    See all Apolipoprotein F primary antibodies
  • Description
    Mouse monoclonal to Apolipoprotein F
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (AAH26257), corresponding to amino acids 37-327 of Human Apolipoprotein F, with a 26kDa enzyme tag.

  • Positive control
    • Human small intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab77452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 3 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • FunctionMinor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo-F antibody
    • Apof antibody
    • APOF_HUMAN antibody
    • Apolipoprotein F antibody
    • DKFZp781G18150 antibody
    • Lipid transfer inhibitor protein antibody
    • LTIP antibody
    • MGC22520 antibody
    see all

Anti-Apolipoprotein F antibody images

  • Anti-Apolipoprotein F antibody (ab77452) at 1/500 dilution + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 34 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Immunoperoxidase staining of Apolipoprotein F in formalin fixed, paraffin embedded human small intestine tissue using ab77452 at 3 µg/ml.

References for Anti-Apolipoprotein F antibody (ab77452)

ab77452 has not yet been referenced specifically in any publications.

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