Anti-Apolipoprotein L 4 antibody (ab175560)

Overview

  • Product name
    Anti-Apolipoprotein L 4 antibody
    See all Apolipoprotein L 4 primary antibodies
  • Description
    Rabbit polyclonal to Apolipoprotein L 4
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Apolipoprotein L 4 aa 267-296 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Surrounding Glutamate 273.
    Database link: Q9BPW4

  • Positive control
    • HL60 cell line lysate; Human testis tissue; HL60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 39 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
  • Tissue specificity
    Widely expressed; the highest levels are in spinal cord, placenta, adrenal gland; also detected in spleen, bone marrow, uterus, trachea, mammary gland and testis; levels are low in brain, heart and pancreas.
  • Sequence similarities
    Belongs to the apolipoprotein L family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • APOL IV antibody
    • ApoL-IV antibody
    • APOL4 antibody
    • APOL4_HUMAN antibody
    • Apolipoprotein L IV antibody
    • Apolipoprotein L-IV antibody
    • Apolipoprotein L4 antibody
    • APOLIV antibody
    • CTA 212A2.2 antibody
    • OTTHUMP00000028937 antibody
    see all

Images

  • Anti-Apolipoprotein L 4 antibody (ab175560) at 1/50 dilution + HL60 cell line lysate at 35 µg

    Predicted band size : 39 kDa
  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human testis tissue labeling Apolipoprotein L 4 with ab175560, at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of HL60 cells labeling Apolipoprotein L 4 with ab175560 at 1/10 (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175560 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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