Anti-Apolipoprotein M antibody (ab76458)


  • Product nameAnti-Apolipoprotein M antibody
    See all Apolipoprotein M primary antibodies
  • Description
    Rabbit polyclonal to Apolipoprotein M
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Apolipoprotein M.

    (Peptide available as ab87119.)

  • Positive control
    • This antibody gave a positive signal in Human Plasma.



Our Abpromise guarantee covers the use of ab76458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).


  • FunctionProbably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
  • Tissue specificityPlasma protein. Expressed in liver and kidney.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family. Highly divergent.
  • Cellular localizationSecreted. Present in high density lipoprotein (HDL) and to a lesser extent in triglyceride-rich lipoproteins (TGRLP) and low density lipoproteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo M antibody
    • Apo-M antibody
    • Apolipoprotein M antibody
    • ApoM antibody
    • APOM_HUMAN antibody
    • G3A antibody
    • HSPC336 antibody
    • MGC22400 antibody
    • NG20 antibody
    • NG20 like protein antibody
    • Protein G3a antibody
    see all

Anti-Apolipoprotein M antibody images

  • Anti-Apolipoprotein M antibody (ab76458) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab76458 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76458, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 cells at 5µg/ml.
  • IHC image of Apolipoprotein M staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-Apolipoprotein M antibody (ab76458)

ab76458 has not yet been referenced specifically in any publications.

Product Wall

Merci de nous avoir contactés.

J’ai vérifié ab76458. Puisque ce produit a une concentration >1mg/ml de BSA, je peux vous confirmer qu’il ne contient pas de BSA. Notez bien que ceci n’est pas le cas p...

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Merci de nous avoir contactés.

ab91656 n’a pas été purifié. Il est vendu sous forme de surnageant et dans un tampon de 49% PBS, 0.01% Azoture de sodium, 50% Glycérol, 0.05% BSA.

ab76458 a &ea...

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Merci de nous avoir contactés.

Comme je vous l’ai expliqué au téléphone, la séquence immunogène d’ab91656 est xxxxxxxx qui correspond aux acides aminés xxxxxxxchez l’humain. J...

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