Anti-Apolipoprotein O antibody [2F1] (ab109757)

Overview

  • Product nameAnti-Apolipoprotein O antibody [2F1]
    See all Apolipoprotein O primary antibodies
  • Description
    Mouse monoclonal [2F1] to Apolipoprotein O
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant Human Apolipoprotein O protein.

  • Positive control
    • HepG2 and 3T3L1 cell lysates; Human testis tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 22 kDa.
ELISA 1/10000.
IHC-P 1/200.

Target

  • FunctionPromotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation.
  • Tissue specificityExpressed in all tissues examined. Up-regulated in diabetic heart.
  • Sequence similaritiesBelongs to the apolipoprotein O family.
  • Post-translational
    modifications
    O-glycosylation; glycosaminoglycan of chondroitin-sulfate type.
  • Cellular localizationMembrane. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610008C08Rik antibody
    • 1110019O03Rik antibody
    • Apolipoprotein O antibody
    • APOO antibody
    • APOO_HUMAN antibody
    • FAM121B antibody
    • Family with sequence similarity 121B antibody
    • MGC130105 antibody
    • MGC130106 antibody
    • MGC4825 antibody
    • My025 antibody
    • MYO25 antibody
    • OTTHUMP00000023073 antibody
    • Protein FAM121B antibody
    • RP23-272D10.2 antibody
    see all

Anti-Apolipoprotein O antibody [2F1] images

  • Immunohistological staining of Human testis, Formalin-Fixed, Paraffin-Embedded tissue, using ab109757 at 1/200 dilution.
  • All lanes : Anti-Apolipoprotein O antibody [2F1] (ab109757) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 3T3L1 cell lysate


    Predicted band size : 22 kDa

References for Anti-Apolipoprotein O antibody [2F1] (ab109757)

ab109757 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (Bis-tris 12%)
Sample Mouse Tissue lysate - other (Brain and liver (total homogenate and purified mit)
Specification Brain and liver (total homogenate and purified mit
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 13 2015

Application Flow Cytometry
Sample Human Cell (lung cancer cell)
Specification lung cancer cell
Preparation Cell harvesting/tissue preparation method: screping and sampling
Sample buffer: 3% BCA blocking buffer, 10%FBS washing buffer
Fixation Paraformaldehyde
Permeabilization No
Username

Ms. joo young chung

Verified customer

Submitted Nov 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"