Anti-Apoptosis inhibitor 5 antibody (ab65836)

Overview

  • Product nameAnti-Apoptosis inhibitor 5 antibody
    See all Apoptosis inhibitor 5 primary antibodies
  • Description
    Rabbit polyclonal to Apoptosis inhibitor 5
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the C-terminus of human Apoptosis inhibitor 5, identical to the related rat and mouse sequence.

  • Positive control
    • Cardiac muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab65836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

Target

  • FunctionAntiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
  • Tissue specificityExpressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.
  • Sequence similaritiesBelongs to the API5 family.
  • DomainTwo regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.
  • Cellular localizationCytoplasm and Nucleus. Cytoplasm. Mainly nuclear. Can also be cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAC 11 antibody
    • AAC-11 antibody
    • AAC11 antibody
    • Antiapoptosis clone 11 protein antibody
    • API 5 antibody
    • API-5 antibody
    • api5 antibody
    • API5 like 1 antibody
    • API5_HUMAN antibody
    • API5L1 antibody
    • Apoptosis inhibitor 5 antibody
    • Cell migration inducing gene 8 protein antibody
    • Cell migration-inducing gene 8 protein antibody
    • Fibroblast growth factor 2 interacting factor 2 antibody
    • Fibroblast growth factor 2-interacting factor antibody
    • FIF antibody
    • MIG 8 antibody
    • MIG8 antibody
    • Migration inducing protein MIG8 antibody
    • Protein XAGL antibody
    see all

Anti-Apoptosis inhibitor 5 antibody images

  • This image shows human epidermal carcinoma tissue stained with ab65836 at 0.5-1µg/ml.
  • Anti-Apoptosis inhibitor 5 antibody (ab65836) at 2 µg/ml + rat cardiac muscle tissue lysate

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ICC/IF image of ab65836 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Apoptosis inhibitor 5 antibody (ab65836)

ab65836 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (retina)
Loading amount 30 µg
Specification retina
Gel Running Conditions Reduced Denaturing (4-20)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Aug 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"