Anti-Apoptosis inhibitor 5 antibody (ab99307)


  • Product nameAnti-Apoptosis inhibitor 5 antibody
    See all Apoptosis inhibitor 5 primary antibodies
  • Description
    Rabbit polyclonal to Apoptosis inhibitor 5
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Immunogen corresponds to a region within amino acids 1-50 of Human Apoptosis inhibitor 5 (XP_508375.2).

  • Positive control
    • Whole cell lysates from HeLa, 293T and Mouse NIH 3T3 cells.



Our Abpromise guarantee covers the use of ab99307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionAntiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
  • Tissue specificityExpressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.
  • Sequence similaritiesBelongs to the API5 family.
  • DomainTwo regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.
  • Cellular localizationCytoplasm and Nucleus. Cytoplasm. Mainly nuclear. Can also be cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAC 11 antibody
    • AAC-11 antibody
    • AAC11 antibody
    • Antiapoptosis clone 11 protein antibody
    • API 5 antibody
    • API-5 antibody
    • api5 antibody
    • API5 like 1 antibody
    • API5_HUMAN antibody
    • API5L1 antibody
    • Apoptosis inhibitor 5 antibody
    • Cell migration inducing gene 8 protein antibody
    • Cell migration-inducing gene 8 protein antibody
    • Fibroblast growth factor 2 interacting factor 2 antibody
    • Fibroblast growth factor 2-interacting factor antibody
    • FIF antibody
    • MIG 8 antibody
    • MIG8 antibody
    • Migration inducing protein MIG8 antibody
    • Protein XAGL antibody
    see all

Anti-Apoptosis inhibitor 5 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling Apoptosis inhibitor 5 with ab99307 at 1/1000 (0.2µg/mg) and 1/200 (1 µg/mg). Detection: DAB.
  • All lanes : Anti-Apoptosis inhibitor 5 antibody (ab99307) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 58 kDa

    Exposure time : 10 seconds
  • ab99307, at 0.4 µg/ml, staining Apoptosis inhibitor 5 in HeLa whole cell lysate immunoprecipitated using ab99307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 1 second.

References for Anti-Apoptosis inhibitor 5 antibody (ab99307)

ab99307 has not yet been referenced specifically in any publications.

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