Anti-Apoptosis repressor with CARD antibody (ab126238)

Overview

  • Product name
    Anti-Apoptosis repressor with CARD antibody
    See all Apoptosis repressor with CARD primary antibodies
  • Description
    Rabbit polyclonal to Apoptosis repressor with CARD
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-197 of Human Apoptosis repressor with CARD (AAH12798).

  • Positive control
    • WB: HeLa lysate ICC/IF: HeLa cells IHC-P: HeLa xenograft; muscle tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Isoform 1 may be involved in RNA splicing.
    Isoform 2 may inhibit apoptosis.
  • Tissue specificity
    Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
  • Sequence similarities
    Contains 1 CARD domain.
  • Cellular localization
    Cytoplasm and Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis repressor with CARD antibody
    • ARC antibody
    • Muscle enriched cytoplasmic protein antibody
    • Muscle-enriched cytoplasmic protein antibody
    • MYC antibody
    • MYP antibody
    • Nol3 antibody
    • NOL3_HUMAN antibody
    • NOP antibody
    • Nop30 antibody
    • Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody
    • Nucleolar protein 3 antibody
    • Nucleolar protein of 30 kDa antibody
    see all

Images

  • Anti-Apoptosis repressor with CARD antibody (ab126238) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 24 kDa
  • Confocal immunofluorescence analysis of ab126238, at a 1/500 dilution, staining Apoptosis repressor with CARD in paraformaldehyde fixed HeLa cells (Green). Alpha-tubulin filaments were labeled with another antibody (Red) at a 1/2000 dilution.
  • ab126238, at a 1/500 dilution, staining Apoptosis repressor with CARD in paraffin embedded muscle tissue by Immunohistochemistry.
  • ab126238, at 1/500 dilution, staining Human Apoptosis repressor wih CARD in paraffin embedded HeLa xenograft by Immunohistochemistry.

References

This product has been referenced in:
  • Li J  et al. SNX13 reduction mediates heart failure through degradative sorting of apoptosis repressor with caspase recruitment domain. Nat Commun 5:5177 (2014). Read more (PubMed: 25295779) »

See 1 Publication for this product

Customer reviews and Q&As

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I've received the fixation and permeabilization protocol from the lab:

1.Add 500µl of 4% paraformaldehyde/PBS into ...

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