Anti-Apoptosis repressor with CARD antibody (ab2002)

Overview

  • Product nameAnti-Apoptosis repressor with CARD antibody
    See all Apoptosis repressor with CARD primary antibodies
  • Description
    Rabbit polyclonal to Apoptosis repressor with CARD
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Apoptosis repressor with CARD aa 191-208 (C terminal).
    Sequence:

    PDPEPEPDFEERDESEDS


    (Peptide available as ab8371)

  • Positive control
    • HeLa whole cell lysate
  • General notesApoptosis Repressor with CARD domain

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab2002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesIHC-P: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 25 kDa. Can be blocked with ARC (C-term) peptide (human).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIsoform 1 may be involved in RNA splicing.
      Isoform 2 may inhibit apoptosis.
    • Tissue specificityHighly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
    • Sequence similaritiesContains 1 CARD domain.
    • Cellular localizationCytoplasm and Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptosis repressor with CARD antibody
      • ARC antibody
      • Muscle enriched cytoplasmic protein antibody
      • Muscle-enriched cytoplasmic protein antibody
      • MYC antibody
      • MYP antibody
      • Nol3 antibody
      • NOL3_HUMAN antibody
      • NOP antibody
      • Nop30 antibody
      • Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody
      • Nucleolar protein 3 antibody
      • Nucleolar protein of 30 kDa antibody
      see all

    Anti-Apoptosis repressor with CARD antibody images

    • All lanes : Anti-Apoptosis repressor with CARD antibody (ab2002) at 1/1000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : KB whole cell lysate
      Lane 3 : A549 whole cell lysate


      Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry of Apoptosis repressor with CARD in human heart tissue with Anti-Apoptosis repressor with CARD (ab2002) at 5 µg/ml.
    • Immunohistochemistry of Apoptosis repressor with CARD in skeletal muscle with Anti-Apoptosis repressor with CARD (ab2002) at 5 µg/ml.

    References for Anti-Apoptosis repressor with CARD antibody (ab2002)

    This product has been referenced in:
    • Razorenova OV  et al. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol 34:739-51 (2014). WB, IHC-P ; Human . Read more (PubMed: 24344197) »

    See 1 Publication for this product

    Product Wall

    Thank you for forwarding this enquiry.

    Reviewing the IHC images from the datasheet, I understood the customers concerns and contacted the testing laboratory.The images were provided tothem by a collaborator who uses red dyes for detection. I...

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    Thank you for your enquiry.

    Based on the customers question I would define ab2002Anti-Apoptosis repressor with CARD antibodyas specific to ARC rather than NOP30. Looking at the two isoforms on UniProt the antibody is specific to isoform 2 as...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"