Anti-Apoptosis repressor with CARD antibody (ab62149)

Overview

  • Product nameAnti-Apoptosis repressor with CARD antibody
    See all Apoptosis repressor with CARD primary antibodies
  • Description
    Rabbit polyclonal to Apoptosis repressor with CARD
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    SPSPTSRKGTSPKIPEGQ

    , corresponding to amino acids 191-208 of Human Apoptosis repressor with CARD

  • Positive control
    • HeLa whole cell lysate. KB whole cell lysate. A549 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000.

Target

Anti-Apoptosis repressor with CARD antibody images

  • All lanes : Anti-Apoptosis repressor with CARD antibody (ab62149) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : KB whole cell lysate
    Lane 3 : A549 whole cell lysate


    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
  • ab62149 at 181 ug/ml, staining Human Apoptosis repressor with CARD in heart tissue by IHC-P.
  • ab62149 at 5µg/ml, staining Human Apoptosis repressor with CARD in skeletal muscle by IHC-P.

References for Anti-Apoptosis repressor with CARD antibody (ab62149)

ab62149 has not yet been referenced specifically in any publications.

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The immunogen is taken from isoform 2, the link is http://www.uniprot.org/uniprot/O60936 and is predicted to be 23 kD. We are working to update our datasheet to show the correct information.

I hope thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"