Overview

  • Product nameApoptotic Cell Enrichment Kit
  • Tests
    30 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    1h 00m
  • Product overview

    Abcam's Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V/magnetic beads (MagBeads). Annexin V is a Ca2+-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS becomes available for binding to annexin V and any of its conjugates. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables separation of apoptotic cells from living cells. The apoptotic cells bound to the MagBeads adhere to the magnet, while non-apoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from healthy cell culture.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsSuitable for: FM, Functional Studiesmore details

Properties

  • RelevanceCell isolation is a way of dissociating cells from tissues and separating the various types in order to obtain a relatively homogeneous population of cells for analysis.

Applications

Our Abpromise guarantee covers the use of ab65782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.

Protocols

References for Apoptotic Cell Enrichment Kit (ab65782)

ab65782 has not yet been referenced specifically in any publications.

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Thank you for your inquiry.

I am happy to confirm that both magnetic separators should be suitable. We recommend the magnetic separator BioVision, Cat. 1999-1.

I can also confirm that the size of the beads ranges from 1 - 7µm....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"