Overview

  • Product name
    Apoptotic DNA Ladder Isolation Kit
    See all DNA fragments kits
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay type
    Direct
  • Product overview

    Apoptotic DNA Ladder Isolation Kit (ab65627) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells and tissues. The new procedure selectively extracts DNA ladders without interference of intact genomic DNA in only 2-4 hours. As little as 5% of apoptotic cells in a sample can be visualized with this high sensitivity assay.



    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab65627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Functional Assay: ab65627 Apoptotic DNA Ladder Isolation Kit
    Functional Assay: ab65627 Apoptotic DNA Ladder Isolation Kit

    Apoptosis induced in Jurkat cells (1e6 cell x mL-1) by incubation for 24 hours with 1) Control medium; 2) 2 uM Camptothecin (ab120115); 3) 10 uM Camptothecin; 4) 10 uM Camptothecin + 20 nM caspase inhibitor Z-VAD(OMe)-FMK (zVAD; ab120487); 5) 50 ng x mL-1 anti-Fas Ab (MBL), 6) 0.05 mM H2O2 (Sigma); 7) 1 mM H2O2. 5 uL of DNA preparation loaded into each well.

  • - Apoptotic DNA Ladder Isolation Kit (ab65627)
    - Apoptotic DNA Ladder Isolation Kit (ab65627)

    DNA ladders were extracted from 2 x 106 Jurkat cells were treated with 2 μM Camptothecin. The kit extracts laddered DNA only but not intact genomic DNA; therefore, more cells can be extracted and load on the agarose gel. Lane 1: 1 kb DNA ladder; Lane 2: 6 hour induction; Lane 3: 12 hour induction.

Protocols

References

This product has been referenced in:
  • Mochizuki S  et al. Effect of ADAM28 on carcinoma cell metastasis by cleavage of von Willebrand factor. J Natl Cancer Inst 104:906-22 (2012). Mouse . Read more (PubMed: 22636800) »

See 1 Publication for this product

Publishing research using ab65627? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

i followed the protocol and didn't add any additional loading buffer to my samples, but the 20 ul sample I loaded just floated up out of the well. the markers worked perfectly. what should I do to make my sample run?

Thank you for contacting us. Please find below the reply from the lab.


The DNA suspension buffer already contains glycerol to help keep the sample in the well. The electric current should not be started immediately after adding the samp...

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We received Apoptotic DNA Ladder Isolation Kit (ab65627) on 4 degree This kit have enzymes So, Our user think that delivery condtion should be -20 Would you please explain this situation ? I look forwad to hearing from you soon

Thank you very much for your email. All Abcam products are thawed to room temperature before shipping and all are shipped out at room temperature every day of the week. Please note that the majority of enzymes are stable at room temperature (RT...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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