Apoptotic DNA Ladder Isolation Kit (ab65627)

Overview

• Product nameApoptotic DNA Ladder Isolation Kit
  See all DNA fragments kits
• Sample type
  Tissue, Adherent cells, Suspension cells
• Assay typeDirect
• Product overview

  Apoptotic DNA Ladder Isolation Kit (ab65627) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells and tissues. The new procedure selectively extracts DNA ladders without interference of intact genomic DNA in only 2-4 hours. As little as 5% of apoptotic cells in a sample can be visualized with this high sensitivity assay.

  
  

  
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• Notes

  Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.

• Tested applicationsSuitable for: Functional Studiesmore details

Properties

• Storage instructionsStore at -20°C. Please refer to protocols.

• Components	Identifier	50 tests
  Ammonium Acetate Solution	Yellow	1 x 250µl
  DNA Ladder Extraction Buffer	WM	1 x 12.5ml
  DNA Suspension Buffer	Green	1 x 2ml
  Enzyme A Solution	Blue	1 x 250µl
  Enzyme B (Lyophilized)	Red	1 vial

• Research areas
  • Kits/ Lysates/ Other
  • Kits
  • Molecular Biology Kits
  • DNA Isolation Kits

  • Kits/ Lysates/ Other
  • Kits
  • Apoptosis Kits
  • DNA Fragmentation
  • DNA ladder

Apoptotic DNA Ladder Isolation Kit images

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  Functional Assay: ab65627 Apoptotic DNA Ladder Isolation Kit

  Apoptosis induced in Jurkat cells (1e6 cell x mL^-1) by incubation for 24 hours with 1) Control medium; 2) 2 uM Camptothecin (ab120115); 3) 10 uM Camptothecin; 4) 10 uM Camptothecin + 20 nM caspase inhibitor Z-VAD(OMe)-FMK (zVAD; ab120487); 5) 50 ng x mL^-1 anti-Fas Ab (MBL), 6) 0.05 mM H₂O₂ (Sigma); 7) 1 mM H2O2. 5 uL of DNA preparation loaded into each well.

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  - Apoptotic DNA Ladder Isolation Kit (ab65627)

  DNA ladders were extracted from 2 x 10⁶ Jurkat cells were treated with 2 μM Camptothecin. The kit extracts laddered DNA only but not intact genomic DNA; therefore, more cells can be extracted and load on the agarose gel. Lane 1: 1 kb DNA ladder; Lane 2: 6 hour induction; Lane 3: 12 hour induction.