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Read our guarantee »Overview
Product name
ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit
See all ApoTrack™ Cytochrome c Apoptosis products (2) ...
Sample type
Adherent cells
Assay type
Direct
Cross reactivity
Reacts with
Mouse, Rat, Human
Product overview
Apoptotic cells which have released mitochondrial cytochrome c into the cytosol can be differentiated from non apoptotic cells which still retain cytochrome c in their mitochondria by fluorescence microscopy.
ab110417 (MSA07) contains a cytochrome c monoclonal antibody (50 µg) and an isotype-specific secondary antibody (50 µg) which has a fluorescein fluorophore conjugated to it. The kit also contains an ATP synthase V subunit alpha monoclonal antibody (50 µg) and the appropriate isotype-specific secondary antibody (50 µg) which has a Texas Red fluorophore conjugated to it.
ATP synthase is an inner mitochondrial membrane protein and serves as a mitochondrial marker since it is not released into the cytoplasm during apoptosis, unlike cytochrome c. Thus one is able to observe cytochrome c and a mitochondrial marker (ATP synthase subunit alpha) simultaneously by fluorescence microscopy using ab110417. Buffers and blocking solution for immunocytochemical analysis are also included in the kit.
Notes
Store Goat Serum at -20°C. Store all other components at 4°C.
Tested applications
ICC/IFmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 200 µg |
|---|---|---|
| 100% normal goat serum | 1 x 20ml | |
| Anti-Complex Va monoclonal antibody | clone 15H4C4 | 1 x 50µg |
| Anti-Cytochrome c monoclonal antibody | clone 37BA11 | 1 x 50µg |
| Buffer (10X PBS, pH 7.4) | 1 x 100ml | |
| Detergent (0.1% (v/v) Triton® X-100 in PBS) | 1 x 30ml | |
| Goat anti-mouse IgG2a - FITC secondary antibody | 1 x 50µg | |
| Goat anti-mouse IgG2b - TXRD secondary antibody | 1 x 50µg |
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Other Apoptosis Kits
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Cell Biology >> Apoptosis >> Mitochondrial
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Immunocytochemistry/ Immunofluorescence - ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit (ab110417)
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Alternative names
- MSA07
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit images:
Immunocytochemistry/ Immunofluorescence - ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit (ab110417)
Protocols
References for ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit (ab110417)
This product has been referenced in:
- Zhang Y et al. The mitochondrial pathway of anesthetic isoflurane-induced apoptosis. J Biol Chem 285:4025-37 (2010).Read more (PubMed: 20007710) »
- Wood-Kaczmar A et al. PINK1 is necessary for long term survival and mitochondrial function in human dopaminergic neurons. PLoS One 3:e2455 (2008).Read more (PubMed: 18560593) »
See all 4 publications for this product
Publishing research using ab110417? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"