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Product name
ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail
See all ApoTrack™ Cytochrome c Apoptosis products (2) ...
Assay type
Quantitative
Cross reactivity
Reacts with
Human
Product overview
The permeabilization of mitochondrial outer membrane and the subsequent release of cytochrome c and other apoptogenic proteins from mitochondrial intermembrane space into the cytoplasm is considered a hallmark of many apoptotic pathways. Therefore assaying these proteins in mitochondrial and cytoplasmic fractions is of prime interest for many researchers.
ab 110415 (MSA12) is a Western blot antibody cocktail that allows for the detection of cytochrome c in cytoplasmic and mitochondria-containing fractions for determining the proportion of released cytochrome c from mitochondria to the cytoplasm from apoptosis. The kit includes antibodies against a cytoplasmic protein marker, glyceraldehyde-3-phosphodehydrogenase (GAPDH), and 2 mitochondrial markers, pyruvate dehydrogenase subunit E1-alpha (a matrix marker), and ATP synthase subunit alpha (an inner membrane marker). This set of control markers allows for the monitoring and/or optimization of the permeabilization conditions.
Cocktail Antibodies:
Mouse anti Cyt. c monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse anti GAPDH monclonal:
Amount: 5ug
Working concentration: 0.1 µg/ml
Mouse anti PDH-E1-alpha monoclonal:
Amount: 100ug
Working concentration: 2 µg/ml
Mouse anti C-V-alpha monoclonal:
Amount: 25ug
Working concentration: 0.5 µg/ml
Notes
Store cocktail at 4°C and control at -80°C
Tested applications
Properties
Storage instructions
Please see Notes section
| Components | 180 µg |
|---|---|
| ab110337 - Human Heart Mitochondria Control | 1 unit |
| Pre-mixed Solution of 4 monoclonal Antibodies | 1 x 180µg |
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Other Apoptosis Kits
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Cell Biology >> Apoptosis >> Mitochondrial
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Western blot - ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 3.6 µg/ml. The antibody cocktail (0.9 mg/ml) should be diluted 250x to a final working concentration of 3.6 µg/ml for Western blotting.
ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail images:
Western blot - ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)
In this experiment, apoptosis was induced in Jurkat and 143B osteosarcoma cells by FAS and also by treatment with staurosporine (HeLa cells were also treated, but only with STS). Mitochondrial and cytoplasmic fractions were isolated (using kit Cell Fractionation Kit ab109719/MS861) and probed using ab110415 (MSA12). As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.
References for ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)
This product has been referenced in:
- Plant PJ et al. Absence of caspase-3 protects against denervation-induced skeletal muscle atrophy. J Appl Physiol 107:224-34 (2009).Read more (PubMed: 19390003) »
- Deas E et al. PINK1 cleavage at position A103 by the mitochondrial protease PARL. Hum Mol Genet 20:867-79 (2011). WB.Read more (PubMed: 21138942) »
See all 2 publications for this product
Publishing research using ab110415? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"