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Apoptotic cells which have released mitochondrial cytochrome c into the cytosol can be differentiated from non apoptotic cells which still retain cytochrome c in their mitochondria by fluorescence microscopy.
ab110417 (MSA07) contains a cytochrome c monoclonal antibody (50 µg) and an isotype-specific secondary antibody (50 µg) which has a fluorescein fluorophore conjugated to it. The kit also contains an ATP synthase V subunit alpha monoclonal antibody (50 µg) and the appropriate isotype-specific secondary antibody (50 µg) which has a Texas Red fluorophore conjugated to it.
ATP synthase is an inner mitochondrial membrane protein and serves as a mitochondrial marker since it is not released into the cytoplasm during apoptosis, unlike cytochrome c. Thus one is able to observe cytochrome c and a mitochondrial marker (ATP synthase subunit alpha) simultaneously by fluorescence microscopy using ab110417. Buffers and blocking solution for immunocytochemical analysis are also included in the kit.
Store Goat Serum at -20°C. Store all other components at 4°C.
|100% normal goat serum||1 x 20ml|
|Anti-Complex Va monoclonal antibody||clone 15H4C4||1 x 50µg|
|Anti-Cytochrome c monoclonal antibody||clone 37BA11||1 x 50µg|
|Buffer (10X PBS, pH 7.4)||1 x 100ml|
|Detergent (0.1% (v/v) Triton® X-100 in PBS)||1 x 30ml|
|Goat anti-mouse IgG2a - FITC secondary antibody||1 x 50µg|
|Goat anti-mouse IgG2b - TXRD secondary antibody||1 x 50µg|
Our Abpromise guarantee covers the use of ab110417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|