Overview

  • Product name
    ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit
    See all ApoTrack™ Cytochrome c Apoptosis kits
  • Detection method
    Fluorescent
  • Sample type
    Adherent cells
  • Assay type
    Direct
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    Apoptotic cells which have released mitochondrial cytochrome c into the cytosol can be differentiated from non apoptotic cells which still retain cytochrome c in their mitochondria by fluorescence microscopy.

    ab110417 (MSA07) contains a cytochrome c monoclonal antibody (50 µg) and an isotype-specific secondary antibody (50 µg) which has a fluorescein fluorophore conjugated to it. The kit also contains an ATP synthase V subunit alpha monoclonal antibody (50 µg) and the appropriate isotype-specific secondary antibody (50 µg) which has a Texas Red fluorophore conjugated to it.

    ATP synthase is an inner mitochondrial membrane protein and serves as a mitochondrial marker since it is not released into the cytoplasm during apoptosis, unlike cytochrome c. Thus one is able to observe cytochrome c and a mitochondrial marker (ATP synthase subunit alpha) simultaneously by fluorescence microscopy using ab110417. Buffers and blocking solution for immunocytochemical analysis are also included in the kit.

  • Notes

    Store Goat Serum at -20°C. Store all other components at 4°C.

  • Tested applications
    Suitable for: ICC/IFmore details
  • Platform
    Reagents

Properties

Applications

Our Abpromise guarantee covers the use of ab110417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:
  • Ismail AM  et al. Extra virgin olive oil potentiates the effects of aromatase inhibitors via glutathione depletion in estrogen receptor-positive human breast cancer (MCF-7) cells. Food Chem Toxicol 62:817-24 (2013). Human . Read more (PubMed: 24161488) »
  • Napolitano M  et al. 17ß-Estradiol protects cerebellar granule cells against ß-amyloid-induced toxicity via the apoptotic mitochondrial pathway. Neurosci Lett N/A:N/A (2013). Read more (PubMed: 24287378) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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