ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail (ab110415)

Overview

  • Product name
    ApoTrack™ Cytochrome c Apoptosis WB Antibody Cocktail
    See all ApoTrack™ Cytochrome c Apoptosis kits
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    The permeabilization of mitochondrial outer membrane and the subsequent release of cytochrome c and other apoptogenic proteins from mitochondrial intermembrane space into the cytoplasm is considered a hallmark of many apoptotic pathways. Therefore assaying these proteins in mitochondrial and cytoplasmic fractions is of prime interest for many researchers.


    ab 110415 (MSA12) is a Western blot antibody cocktail that allows for the detection of cytochrome c in cytoplasmic and mitochondria-containing fractions for determining the proportion of released cytochrome c from mitochondria to the cytoplasm from apoptosis. The kit includes antibodies against a cytoplasmic protein marker, glyceraldehyde-3-phosphodehydrogenase (GAPDH), and 2 mitochondrial markers, pyruvate dehydrogenase subunit E1-alpha (a matrix marker), and ATP synthase subunit alpha (an inner membrane marker). This set of control markers allows for the monitoring and/or optimization of the permeabilization conditions.


    Cocktail Antibodies:


    Mouse anti Cyt. c monoclonal:
    Amount: 50 µg
    Working concentration: 1 µg/ml


    Mouse anti GAPDH monclonal:
    Amount: 5ug
    Working concentration: 0.1 µg/ml


    Mouse anti PDH-E1-alpha monoclonal:
    Amount: 100ug
    Working concentration: 2 µg/ml


    Mouse anti C-V-alpha monoclonal:
    Amount: 25ug
    Working concentration: 0.5 µg/ml


     

  • Tested applications
    Suitable for: WBmore details

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 3.6 µg/ml. The antibody cocktail (0.9 mg/ml) should be diluted 250x to a final working concentration of 3.6 µg/ml for Western blotting.

Images

  • In this experiment, apoptosis was induced in Jurkat and 143B osteosarcoma cells by FAS and also by treatment with staurosporine (HeLa cells were also treated, but only with STS). Mitochondrial and cytoplasmic fractions were isolated (using kit Cell Fractionation Kit ab109719/MS861) and probed using ab110415 (MSA12). As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.

References

This product has been referenced in:
  • Estlack LE  et al. Nanosecond pulsed electric fields modulate the expression of Fas/CD95 death receptor pathway regulators in U937 and Jurkat Cells. Apoptosis 19:1755-68 (2014). Read more (PubMed: 25331537) »
  • Alquezar C  et al. Inactivation of CDK/pRb pathway normalizes survival pattern of lymphoblasts expressing the FTLD-progranulin mutation c.709-1G>A. PLoS One 7:e37057 (2012). Read more (PubMed: 22623979) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for contacting Abcam (Mitosciences is now an Abcam company). I have contacted the lab in response to your email, asking about MS502(ab110237) and MS507 (ab14748). Unfortunately the epitopes that that these antibodies bind to have not ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up