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The permeabilization of mitochondrial outer membrane and the subsequent release of cytochrome c and other apoptogenic proteins from mitochondrial intermembrane space into the cytoplasm is considered a hallmark of many apoptotic pathways. Therefore assaying these proteins in mitochondrial and cytoplasmic fractions is of prime interest for many researchers.
ab 110415 (MSA12) is a Western blot antibody cocktail that allows for the detection of cytochrome c in cytoplasmic and mitochondria-containing fractions for determining the proportion of released cytochrome c from mitochondria to the cytoplasm from apoptosis. The kit includes antibodies against a cytoplasmic protein marker, glyceraldehyde-3-phosphodehydrogenase (GAPDH), and 2 mitochondrial markers, pyruvate dehydrogenase subunit E1-alpha (a matrix marker), and ATP synthase subunit alpha (an inner membrane marker). This set of control markers allows for the monitoring and/or optimization of the permeabilization conditions.
Mouse anti Cyt. c monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse anti GAPDH monclonal:
Working concentration: 0.1 µg/ml
Mouse anti PDH-E1-alpha monoclonal:
Working concentration: 2 µg/ml
Mouse anti C-V-alpha monoclonal:
Working concentration: 0.5 µg/ml
Store cocktail at 4°C and control at -80°C
|ab110337 - Human Heart Mitochondria Control||1 unit|
|Pre-mixed Solution of 4 monoclonal Antibodies||1 x 180µg|
Our Abpromise guarantee covers the use of ab110415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 3.6 µg/ml. The antibody cocktail (0.9 mg/ml) should be diluted 250x to a final working concentration of 3.6 µg/ml for Western blotting.|