Anti-APPBP1 antibody - C-terminal (ab71526)

Overview

  • Product nameAnti-APPBP1 antibody - C-terminal
    See all APPBP1 primary antibodies
  • Description
    Rabbit polyclonal to APPBP1 - C-terminal
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human APPBP1 conjugated to KLH.

  • Positive control
    • Human breast carcinoma tissue. Mouse brain tissue lysate. 293 cell lysate transiently transfected with the APPBP1 gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab71526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • FunctionRegulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
  • Tissue specificityUbiquitous in fetal tissues. Expressed throughout the adult brain.
  • PathwayProtein modification; protein neddylation.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family. ULA1 subfamily.
  • Cellular localizationCell membrane. Colocalizes with APP in lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • 59 kDa antibody
    • A 116A10.1 antibody
    • Amyloid beta precursor protein binding protein 1 59kD antibody
    • Amyloid beta precursor protein binding protein 1 59kDa antibody
    • Amyloid beta precursor protein binding protein 1 antibody
    • Amyloid beta precursor protein binding protein 1, 59 kDa antibody
    • Amyloid beta precursor protein-binding protein 1 antibody
    • Amyloid protein binding protein 1 antibody
    • Amyloid protein-binding protein 1 antibody
    • APP BP1 antibody
    • APP-BP1 antibody
    • APPBP 1 antibody
    • APPBP1 antibody
    • HPP 1 antibody
    • HPP1 antibody
    • NAE 1 antibody
    • NAE1 antibody
    • NEDD8 activating enzyme E1 regulatory subunit antibody
    • NEDD8 activating enzyme E1 subunit 1 antibody
    • NEDD8 activating enzyme E1 subunit antibody
    • NEDD8-activating enzyme E1 regulatory subunit antibody
    • Proto oncogene protein 1 antibody
    • Proto-oncogene protein 1 antibody
    • Protooncogene protein 1 antibody
    • ula 1 antibody
    • ULA1_HUMAN antibody
    see all

Anti-APPBP1 antibody - C-terminal images

  • Anti-APPBP1 antibody - C-terminal (ab71526) at 1/60 dilution + mouse brain tissue lysate at 35 µg

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-APPBP1 antibody - C-terminal (ab71526) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the APPBP1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.
  • ab71526, at 1/50 dilution, staining APPBP1 in human breast carcinoma by immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by a peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-APPBP1 antibody - C-terminal (ab71526)

ab71526 has not yet been referenced specifically in any publications.

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