Anti-APPBP1 antibody - Carboxyterminal end (ab71592)

Overview

  • Product name
    Anti-APPBP1 antibody - Carboxyterminal end
    See all APPBP1 primary antibodies
  • Description
    Rabbit polyclonal to APPBP1 - Carboxyterminal end
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Positive control
    • Formalin-fixed, paraffin-embedded human testis tissue and transfected/ non-transfected 293 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/1000.

Target

  • Function
    Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
  • Tissue specificity
    Ubiquitous in fetal tissues. Expressed throughout the adult brain.
  • Pathway
    Protein modification; protein neddylation.
  • Sequence similarities
    Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.
  • Cellular localization
    Cell membrane. Colocalizes with APP in lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • 59 kDa antibody
    • A 116A10.1 antibody
    • Amyloid beta precursor protein binding protein 1 59kD antibody
    • Amyloid beta precursor protein binding protein 1 59kDa antibody
    • Amyloid beta precursor protein binding protein 1 antibody
    • Amyloid beta precursor protein binding protein 1, 59 kDa antibody
    • Amyloid beta precursor protein-binding protein 1 antibody
    • Amyloid protein binding protein 1 antibody
    • Amyloid protein-binding protein 1 antibody
    • APP BP1 antibody
    • APP-BP1 antibody
    • APPBP 1 antibody
    • APPBP1 antibody
    • HPP 1 antibody
    • HPP1 antibody
    • NAE 1 antibody
    • NAE1 antibody
    • NEDD8 activating enzyme E1 regulatory subunit antibody
    • NEDD8 activating enzyme E1 subunit 1 antibody
    • NEDD8 activating enzyme E1 subunit antibody
    • NEDD8-activating enzyme E1 regulatory subunit antibody
    • Proto oncogene protein 1 antibody
    • Proto-oncogene protein 1 antibody
    • Protooncogene protein 1 antibody
    • ula 1 antibody
    • ULA1_HUMAN antibody
    see all

Images

  • All lanes : Anti-APPBP1 antibody - Carboxyterminal end (ab71592) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysates
    Lane 2 : APPBP1 transfected 293 cell lysates

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • Formalin-fixed and paraffin-embedded human testis tissue reacted with ab71592 at a 1:10 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References

ab71592 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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