Anti-APPBP1 antibody - N-terminal (ab71542)


  • Product nameAnti-APPBP1 antibody - N-terminal
    See all APPBP1 primary antibodies
  • Description
    Rabbit polyclonal to APPBP1 - N-terminal
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human APPBP1 conjugated to KLH.

  • Positive control
    • APPBP1-transfected 293 cells lysate; human brain tissue.



Our Abpromise guarantee covers the use of ab71542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/50 - 1/100. Predicted molecular weight: 60 kDa.
IHC-P 1/10 - 1/50.


  • FunctionRegulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
  • Tissue specificityUbiquitous in fetal tissues. Expressed throughout the adult brain.
  • PathwayProtein modification; protein neddylation.
  • Sequence similaritiesBelongs to the ubiquitin-activating E1 family. ULA1 subfamily.
  • Cellular localizationCell membrane. Colocalizes with APP in lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • 59 kDa antibody
    • A 116A10.1 antibody
    • Amyloid beta precursor protein binding protein 1 59kD antibody
    • Amyloid beta precursor protein binding protein 1 59kDa antibody
    • Amyloid beta precursor protein binding protein 1 antibody
    • Amyloid beta precursor protein binding protein 1, 59 kDa antibody
    • Amyloid beta precursor protein-binding protein 1 antibody
    • Amyloid protein binding protein 1 antibody
    • Amyloid protein-binding protein 1 antibody
    • APP BP1 antibody
    • APP-BP1 antibody
    • APPBP 1 antibody
    • APPBP1 antibody
    • HPP 1 antibody
    • HPP1 antibody
    • NAE 1 antibody
    • NAE1 antibody
    • NEDD8 activating enzyme E1 regulatory subunit antibody
    • NEDD8 activating enzyme E1 subunit 1 antibody
    • NEDD8 activating enzyme E1 subunit antibody
    • NEDD8-activating enzyme E1 regulatory subunit antibody
    • Proto oncogene protein 1 antibody
    • Proto-oncogene protein 1 antibody
    • Protooncogene protein 1 antibody
    • ula 1 antibody
    • ULA1_HUMAN antibody
    see all

Anti-APPBP1 antibody - N-terminal images

  • All lanes : Anti-APPBP1 antibody - N-terminal (ab71542) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the APPBP1 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size : 60 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Analysis of APPBP1 expression in formalin-fixed, paraffin embedded human brain tissue using ab71542, at 1/10 dilution (peroxidase-conjugated secondary antibody; DAB staining).

References for Anti-APPBP1 antibody - N-terminal (ab71542)

ab71542 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab71542.
Please use the links above to contact us or submit feedback about this product.