Overview

  • Product nameAnti-APPBP2 antibody
    See all APPBP2 primary antibodies
  • Description
    Mouse monoclonal to APPBP2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Zebrafish, Orangutan
  • Immunogen

    Recombinant fragment: NQYENAEKLY LRSIAIGKKL FGEGYSGLEY DYRGLIKLYN SIGNYEKVFE YHNVLSNWNR LRDRQYSVTD ALEDVSTSPQ STEEVVQSFL ISQNVEGPSC, corresponding to amino acids 486-585 of APPBP2 (NP_006371) with a 26 kDa tag.

  • Positive control
    • APPBP2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 67 kDa.
ELISA Use at an assay dependent dilution.

Target

Anti-APPBP2 antibody images

  • Anti-APPBP2 antibody (ab77732) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 67 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-APPBP2 antibody (ab77732) at 5 µg/ml

    Lane 1 : APPBP2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 67 kDa
    Observed band size : 67 kDa

References for Anti-APPBP2 antibody (ab77732)

ab77732 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"