Overview

  • Product nameAnti-APPD antibody
    See all APPD primary antibodies
  • Description
    Rabbit polyclonal to APPD
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 243-257 of Human APPD.

  • Positive control
    • HeLa whole cell lysate.
  • General notes


    APPD is predicted through gene annotation and contains pleckstrin homology domain. It may binds to two Zn++ ions through its FYVE zinc finger domain. Biological function of this protein has not been clearly mapped yet.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, Sucrose
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesAPPD is predicted through gene annotation and contains pleckstrin homology domain. It may binds to two Zn++ ions through its FYVE zinc finger domain. Biological function of this protein has not been clearly mapped yet.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 33.5 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8.
  • Tissue specificityHighly expressed in heart and skeletal muscle. Weakly expressed in brain, thymus, spleen, kidney, liver, small intestine, placenta and lung.
  • Sequence similaritiesContains 1 FYVE-type zinc finger.
    Contains 1 PH domain.
  • DomainPH and FYVE-type zinc finger domains are required for lysosomal location.
  • Cellular localizationNucleus. Cytoplasm > perinuclear region. Lysosome. Translocates to lysosome during apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis inducing protein antibody
    • Apoptosis inducing protein D antibody
    • Apoptosis-inducing protein antibody
    • LAPF antibody
    • Lysosome associated apoptosis inducing protein containing PH and FYVE domains antibody
    • Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains antibody
    • MGC4090 antibody
    • PH and FYVE domain containing protein 1 antibody
    • PH and FYVE domain-containing protein 1 antibody
    • PH domain containing family F member 1 antibody
    • PH domain-containing family F member 1 antibody
    • Phafin 1 antibody
    • Phafin-1 antibody
    • Phafin1 antibody
    • PKHF1_HUMAN antibody
    • Pleckstrin homology domain containing family F (with FYVE domain) member 1 antibody
    • Pleckstrin homology domain containing family F member 1 antibody
    • Pleckstrin homology domain containing family F with FYVE domain member 1 antibody
    • Pleckstrin homology domain-containing family F member 1 antibody
    • PLEKHF 1 antibody
    • Plekhf1 antibody
    • ZFYVE 15 antibody
    • ZFYVE15 antibody
    • Zinc finger FYVE domain containing protein 15 antibody
    • Zinc finger FYVE domain-containing protein 15 antibody
    see all

Anti-APPD antibody images



  • Predicted band size : 33.5 kDa


    ab10081 at 1/1000 dilution staining approximately 31kDa band of human APPD in Hela whole cell lysate by Western blot (ECL).

References for Anti-APPD antibody (ab10081)

ab10081 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"