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Rabbit polyclonal to APPL - N-terminal
HumanPredicted to work with:
Mouse, Cow, Pig, Xenopus laevis, Zebrafish
Recombinant fragment, corresponding to a region within N-terminal amino acids 1-205 of Human APPL (UniProt: Q9UKG1).
- U-87 MG and MCF7 whole cell lysates; Human AZ521 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 80 kDa.
||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
High levels in heart, ovary, pancreas and skeletal muscle.
Contains 1 PH domain.
Contains 1 PID domain.
Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
Information by UniProt
- Adapter protein containing PH domain antibody
- Adaptor Protein Containing pH Domain antibody
- Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
Western blot - Anti-APPL antibody (ab153809)
Anti-APPL antibody - N-terminal (ab153809) at 1/1000 dilution + MCF7 whole cell
lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody (ab153809)
Immunohistochemical analysis of paraffin-embedded Human AZ521 xenograft tissue labeling APPL with ab153809 at 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"