Anti-APPL antibody - N-terminal (ab153809)

Overview

  • Product name
    Anti-APPL antibody - N-terminal
    See all APPL primary antibodies
  • Description
    Rabbit polyclonal to APPL - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within N-terminal amino acids 1-205 of Human APPL (UniProt: Q9UKG1).

  • Positive control
    • U-87 MG and MCF7 whole cell lysates; Human AZ521 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 80 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
  • Tissue specificity
    High levels in heart, ovary, pancreas and skeletal muscle.
  • Sequence similarities
    Contains 1 PH domain.
    Contains 1 PID domain.
  • Domain
    Overexpression of an N-terminal domain (residues 1-319) or a C-terminal region (residues 273-709) has a proapoptotic effect.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter protein containing PH domain antibody
    • Adaptor Protein Containing pH Domain antibody
    • Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody
    • Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody
    • Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1 antibody
    • AKT2 INTERACTOR antibody
    • APPL 1 antibody
    • Appl1 antibody
    • DCC interacting protein 13 alpha antibody
    • DCC-interacting protein 13-alpha antibody
    • DIP 13 alpha antibody
    • DIP13 alpha antibody
    • Dip13-alpha antibody
    • DIP13alpha antibody
    • DP13A_HUMAN antibody
    • KIAA1428 antibody
    • PTB domain and leucine zipper motif 1 antibody
    • PTB Domain and Leucine Zipper Motif antibody
    • Signaling adaptor protein DIP13alpha antibody
    see all

Images

  • Anti-APPL antibody - N-terminal (ab153809) at 1/1000 dilution + MCF7 whole cell
    lysate at 30 µg

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human AZ521 xenograft tissue labeling APPL with ab153809 at 1/100 dilution.

References

ab153809 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (12.5%)
Sample
Mouse Cell lysate - whole cell (Hep G2)
Specification
Hep G2
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

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Verified customer

Submitted Jan 22 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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