Recombinant
RabMAb

Anti-Aprataxin antibody [EPR8751] (ab192598)

Overview

  • Product name
    Anti-Aprataxin antibody [EPR8751]
    See all Aprataxin primary antibodies
  • Description
    Rabbit monoclonal [EPR8751] to Aprataxin
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Aprataxin aa 100-200. The exact sequence is proprietary.
    Database link: Q7Z2E3

  • Positive control
    • Human cerebellum and Human fetal brain lysates; HeLa, A549 and 293 cell lysates. Human cervix carcinoma tissue. HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR8751
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/200.

Target

  • Function
    DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity.
  • Tissue specificity
    Widely expressed. In brain, it is expressed in the posterior cortex, cerebellum, hippocampus and olfactory bulb. Isoform 1 is highly expressed in the cerebral cortex and cerebellum, compared to isoform 2.
  • Involvement in disease
    Defects in APTX are the cause of ataxia-oculomotor apraxia syndrome (AOA) [MIM:208920]. AOA is an autosomal recessive syndrome characterized by early-onset cerebellar ataxia, oculomotor apraxia, early areflexia and late peripheral neuropathy.
    Defects in APTX are a cause of coenzyme Q10 deficiency (COQ10D) [MIM:607426]. Coenzyme Q10 deficiency is an autosomal recessive disorder with variable manifestations. It can be associated with three main clinical phenotypes: a predominantly myopathic form with central nervous system involvement, an infantile encephalomyopathy with renal dysfunction and an ataxic form with cerebellar atrophy.
  • Sequence similarities
    Contains 1 C2H2-type zinc finger.
    Contains 1 FHA-like domain.
    Contains 1 HIT domain.
  • Domain
    The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
    The FHA-like domain mediates interaction with NCL; XRCC1 and XRCC4.
    The HIT domain is required for enzymatic activity.
    The C2H2-type zinc finger mediates DNA-binding.
  • Cellular localization
    Nucleus > nucleoplasm. Nucleus > nucleolus. Upon genotoxic stress, colocalizes with XRCC1 at sites of DNA damage. Colocalizes with MDC1 at sites of DNA double-strand breaks. Interaction with NCL is required for nucleolar localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • AOA 1 antibody
    • AOA antibody
    • AOA1 antibody
    • Aprataxin antibody
    • Aprataxin homolog antibody
    • Aptx antibody
    • APTX_HUMAN antibody
    • Ataxia 1 early onset with hypoalbuminemia antibody
    • Ataxia1 early onset with hypoalbuminemia antibody
    • AXA 1 antibody
    • AXA1 antibody
    • EAOH antibody
    • EOAHA antibody
    • FHA HIT antibody
    • FHA-HIT antibody
    • FLJ20157 antibody
    • Forkhead associated domain histidine triad like antibody
    • Forkhead associated domain histidine triad like protein antibody
    • Forkhead-associated domain histidine triad-like protein antibody
    • MGC1072 antibody
    see all

Images

  • All lanes : Anti-Aprataxin antibody [EPR8751] (ab192598) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : Hman cerebellum lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • Anti-Aprataxin antibody [EPR8751] (ab192598) at 1/2000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 40 kDa
    Additional bands at : 29-40 kDa (possible isoform).

    Identify seven isoforms

  • Immunohistochemical analysis of paraffin-embedded, Human cervix carcinoma tissue labeling Aprataxin with ab192598 at a 1/1000 dilution. Counter stained with hematoxylin. Negative control also shown.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling Aprataxin with ab192598 at a 1/200 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.

References

ab192598 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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