The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant Human APRIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution). Allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human APRIL
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to APRIL (Biotin) (ab84029). ab84029 can be used as the detection antibody and ab109566 as the capture antibody. Detection limit for recombinant Human APRIL: 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
FunctionCytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
Tissue specificityExpressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
Sequence similaritiesBelongs to the tumor necrosis factor family.
Post-translational modificationsThe precursor is cleaved by furin.