Overview

  • Product name
  • Description
    Rabbit polyclonal to APRIL
  • Specificity
    This antibody detects endogenous levels of total APRIL protein.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human APRIL aa 151-200. The exact sequence is proprietary.
    Sequence:

    QGYGVRIQDA GVYLLYSQVL FQDVTFTMGQ VVSREGQGRQ ETLFRCIRSM


    Database link: O75888

  • Positive control
    • Human brain cortex tissue; HeLa and COLO205 cell lysates; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 27 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
  • Tissue specificity
    Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The precursor is cleaved by furin.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A proliferation inducing ligand antibody
    • A proliferation-inducing ligand antibody
    • APRIL antibody
    • CD256 antibody
    • TALL-2 antibody
    • TALL2 antibody
    • TNF related death ligand antibody
    • TNF- and APOL-related leukocyte expressed ligand 2 antibody
    • TNF-related death ligand 1 antibody
    • TNF13_HUMAN antibody
    • TNFSF13 antibody
    • TRDL-1 antibody
    • TRDL1 antibody
    • Tumor necrosis factor (ligand) superfamily, member 13 antibody
    • Tumor necrosis factor ligand superfamily member 13 antibody
    • Tumor necrosis factor like protein ZTNF2 antibody
    • Tumor necrosis factor related death ligand 1 antibody
    • UNQ383/PRO715 antibody
    • ZTNF2 antibody
    see all

Images

  • Immunofluorescence of HeLa cells labeling APRIL using ab189263 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.

  • Immunohistochemical analysis of paraffin embedded formalin fixed human brain cortex tissue labeling APRIL using ab189263 at 10 µg/ml.

  • All lanes : Anti-APRIL antibody (ab189263) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : COLO205 cell lysate
    Lane 3 : COLO205 cell lysate with immunizing peptide


    Predicted band size : 27 kDa

References

ab189263 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab189263.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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