Overview

  • Product nameAnti-APRIL antibody
    See all APRIL primary antibodies
  • Description
    Rabbit polyclonal to APRIL
  • Tested applicationsSuitable for: IHC-Fr, ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal region of human APRIL.

  • Positive control
    • Human colon carcinoma tissue. Extracts from HeLa cells and COLO205 cells. HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21191015
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionCytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
  • Tissue specificityExpressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The precursor is cleaved by furin.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A proliferation inducing ligand antibody
    • A proliferation-inducing ligand antibody
    • APRIL antibody
    • CD256 antibody
    • TALL-2 antibody
    • TALL2 antibody
    • TNF related death ligand antibody
    • TNF- and APOL-related leukocyte expressed ligand 2 antibody
    • TNF-related death ligand 1 antibody
    • TNF13_HUMAN antibody
    • TNFSF13 antibody
    • TRDL-1 antibody
    • TRDL1 antibody
    • Tumor necrosis factor (ligand) superfamily, member 13 antibody
    • Tumor necrosis factor ligand superfamily member 13 antibody
    • Tumor necrosis factor like protein ZTNF2 antibody
    • Tumor necrosis factor related death ligand 1 antibody
    • UNQ383/PRO715 antibody
    • ZTNF2 antibody
    see all

Anti-APRIL antibody images

  • All lanes : Anti-APRIL antibody (ab64967) at 1/500 dilution

    Lane 1 : extracts from HeLa cells without immunising peptide
    Lane 2 : extracts from COLO205 cells, without immunising peptide
    Lane 3 : extracts from COLO205 cells, with immunising peptide


    Predicted band size : 27 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 1/50 ab64967. Left: without immunising peptide. Right: with immunising peptide.
  • Immunofluorescence analysis of HeLa cells, using 1/500 ab64967. Left: without immunising peptide. Right: with immunising peptide.

References for Anti-APRIL antibody (ab64967)

This product has been referenced in:
  • Qian Z  et al. High expression of TNFSF13 in tumor cells and fibroblasts is associated with poor prognosis in non-small cell lung cancer. Am J Clin Pathol 141:226-33 (2014). Human . Read more (PubMed: 24436270) »
  • Phares TW  et al. Factors supporting intrathecal humoral responses following viral encephalomyelitis. J Virol 85:2589-98 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21191015) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"