Overview

  • Product name
  • Description
    Rabbit polyclonal to APRIL
  • Tested applications
    Suitable for: IHC-Fr, ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal region of human APRIL.

  • Positive control
    • Human colon carcinoma tissue. Extracts from HeLa cells and COLO205 cells. HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21191015
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function
    Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
  • Tissue specificity
    Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The precursor is cleaved by furin.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A proliferation inducing ligand antibody
    • A proliferation-inducing ligand antibody
    • APRIL antibody
    • CD256 antibody
    • TALL-2 antibody
    • TALL2 antibody
    • TNF related death ligand antibody
    • TNF- and APOL-related leukocyte expressed ligand 2 antibody
    • TNF-related death ligand 1 antibody
    • TNF13_HUMAN antibody
    • TNFSF13 antibody
    • TRDL-1 antibody
    • TRDL1 antibody
    • Tumor necrosis factor (ligand) superfamily, member 13 antibody
    • Tumor necrosis factor ligand superfamily member 13 antibody
    • Tumor necrosis factor like protein ZTNF2 antibody
    • Tumor necrosis factor related death ligand 1 antibody
    • UNQ383/PRO715 antibody
    • ZTNF2 antibody
    see all

Images

  • All lanes : Anti-APRIL antibody (ab64967) at 1/500 dilution

    Lane 1 : extracts from HeLa cells without immunising peptide
    Lane 2 : extracts from COLO205 cells, without immunising peptide
    Lane 3 : extracts from COLO205 cells, with immunising peptide


    Predicted band size : 27 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 1/50 ab64967. Left: without immunising peptide. Right: with immunising peptide.
  • Immunofluorescence analysis of HeLa cells, using 1/500 ab64967. Left: without immunising peptide. Right: with immunising peptide.

References

This product has been referenced in:
  • Dou H  et al. APRIL, BCMA and TACI proteins are abnormally expressed in non-small cell lung cancer. Oncol Lett 12:3351-3355 (2016). WB, IHC-P ; Human . Read more (PubMed: 27900003) »
  • Osório C  et al. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci 59:24-36 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24444792) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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