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Rabbit polyclonal to APRIL
Predicted to work with:
Synthetic peptide derived from internal region of human APRIL.
Human colon carcinoma tissue. Extracts from HeLa cells and
COLO205 cells. HeLa cells.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21191015
1/500 - 1/1000.
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa).
1/50 - 1/100.
Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
Belongs to the tumor necrosis factor family.
The precursor is cleaved by furin.
Information by UniProt
A proliferation inducing ligand antibody
A proliferation-inducing ligand antibody
Western blot - Anti-APRIL antibody (ab64967)
All lanes :
Anti-APRIL antibody (ab64967) at 1/500 dilution
Lane 1 :
extracts from HeLa cells without immunising peptide
Lane 2 :
extracts from COLO205 cells, without immunising peptide
Lane 3 :
extracts from COLO205 cells, with immunising peptide
Predicted band size :
Observed band size :
24 kDa (
why is the actual band size different from the predicted?
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APRIL antibody (ab64967)
Immunohistochemistry analysis of paraffin-embedded
human colon carcinoma tissue using 1/50 ab64967. Left: without immunising peptide. Right: with immunising peptide.
Immunocytochemistry/ Immunofluorescence - Anti-APRIL antibody (ab64967)
Immunofluorescence analysis of HeLa cells, using 1/500 ab64967. Left: without immunising peptide. Right: with immunising peptide.
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