Overview

  • Product nameAnti-APRIL antibody [Aprily-1]
    See all APRIL primary antibodies
  • Description
    Mouse monoclonal [Aprily-1] to APRIL
  • Tested applicationsSuitable for: IP, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 93 - 233 of Human APRIL, expressed in bacteria.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • ClonalityMonoclonal
  • Clone numberAprily-1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Flow Cyt
WB
  • Application notesFlow Cyt: Use at a concentration of 10 µg/ml. This antibody also works for intracellular FC staining.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
    • Tissue specificityExpressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
    • Sequence similaritiesBelongs to the tumor necrosis factor family.
    • Post-translational
      modifications
      The precursor is cleaved by furin.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • A proliferation inducing ligand antibody
      • A proliferation-inducing ligand antibody
      • APRIL antibody
      • CD256 antibody
      • TALL-2 antibody
      • TALL2 antibody
      • TNF related death ligand antibody
      • TNF- and APOL-related leukocyte expressed ligand 2 antibody
      • TNF-related death ligand 1 antibody
      • TNF13_HUMAN antibody
      • TNFSF13 antibody
      • TRDL-1 antibody
      • TRDL1 antibody
      • Tumor necrosis factor (ligand) superfamily, member 13 antibody
      • Tumor necrosis factor ligand superfamily member 13 antibody
      • Tumor necrosis factor like protein ZTNF2 antibody
      • Tumor necrosis factor related death ligand 1 antibody
      • UNQ383/PRO715 antibody
      • ZTNF2 antibody
      see all

    Anti-APRIL antibody [Aprily-1] images

    • ab16088 at 10ug/ml detecting APRIL by FACS analysis.

      Left hand panel mock transfected cells

      Right hand panel human APRIL tranfected cells

      HEK 293 cells were mock transfected or transfected with an expression plasmid enabling surface expression of a non-cleavable human APRIL. Cells (5x105) were incubated on ice for 30 min in 50 ul FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 10ug/ml of Aprily-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.



    • Predicted band size : 30 kDa


      Western blot of total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-1 reacts specifically with human APRIL.

      10ug of protein was applied to the gel. Revealed with ab16088 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).

      The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).

    References for Anti-APRIL antibody [Aprily-1] (ab16088)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    The immunogen of ab16088 is a recombinant fragment corresponding to amino acids 93 - 233 of Human APRIL.

    The uncleaved protein is 250 amino acids long as is cleaved at amino acid 104-105 into 2 fragments: the propeptide (aa 1-104) and the chai...

    Read More

    Thank you for your email. I am sorry for the delay as lab took more time than anticipated.

    We may be able to provide you the positive control lysates for ab16088. This would be whole cell lysate of HEK 293T cells transfected with an expression...

    Read More

    Thank you for contacting us.

    I can confirm that, I have sent reminder to our collaborator lab. I will send you the requested details as soon as I receive a reply from them.

    Many thanks for having patience!

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"