Anti-APRIL antibody [Sacha-1] (ab16085)

Overview

  • Product nameAnti-APRIL antibody [Sacha-1]
    See all APRIL primary antibodies
  • Description
    Rat monoclonal [Sacha-1] to APRIL
  • Tested applicationsSuitable for: Flow Cyt, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fusion protein, corresponding to amino acids 105 - 250 of Human APRIL fused to amino acids 16 - 108 of human headless, ACRP30.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • ClonalityMonoclonal
  • Clone numberSacha-1
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 1 µg/ml. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC Use a concentration of 5 µg/ml.

Target

  • FunctionCytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
  • Tissue specificityExpressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The precursor is cleaved by furin.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A proliferation inducing ligand antibody
    • A proliferation-inducing ligand antibody
    • APRIL antibody
    • CD256 antibody
    • TALL-2 antibody
    • TALL2 antibody
    • TNF related death ligand antibody
    • TNF- and APOL-related leukocyte expressed ligand 2 antibody
    • TNF-related death ligand 1 antibody
    • TNF13_HUMAN antibody
    • TNFSF13 antibody
    • TRDL-1 antibody
    • TRDL1 antibody
    • Tumor necrosis factor (ligand) superfamily, member 13 antibody
    • Tumor necrosis factor ligand superfamily member 13 antibody
    • Tumor necrosis factor like protein ZTNF2 antibody
    • Tumor necrosis factor related death ligand 1 antibody
    • UNQ383/PRO715 antibody
    • ZTNF2 antibody
    see all

Anti-APRIL antibody [Sacha-1] images

  • ab16085 at 1ug/ml detecting  membrane-bound human APRIL on HEK 293T cells by FACS analysis.

     

    HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of a non-cleavable mouse APRIL (thick line). Cells were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1ug/ml of MAb to APRIL (Sacha-1). After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.

  • Immunostaining of HEK-293T cells transfected with a murine APRIL expression plasmid (left panels), or mock transfected (right panel).

    Three days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5ug/ml ab16085 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

References for Anti-APRIL antibody [Sacha-1] (ab16085)

ab16085 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. I am sorry for the delay as lab took more time than anticipated.

We may be able to provide you the positive control lysates for ab16088. This would be whole cell lysate of HEK 293T cells transfected with an expression...

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Thank you for contacting us.

I can confirm that, I have sent reminder to our collaborator lab. I will send you the requested details as soon as I receive a reply from them.

Many thanks for having patience!

Thank you for your enquiry and patience. Regarding ab16085 (APRIL antibody), this antibody has not been tested on endogeneous APRIL. This would be difficult because APRIL is rapidly cleaved in the cells by furin and secreted. ab16085 was tested in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"