Overview

  • Product name
  • Description
    Mouse polyclonal to APRT
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    APRT (NP_000476.1, 1 a.a. ~ 180 a.a) full-length human protein.

  • Positive control
    • Human liver lysate. APRT transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa).

Target

  • Function
    Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
  • Pathway
    Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
  • Involvement in disease
    Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:102600]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.
  • Sequence similarities
    Belongs to the purine/pyrimidine phosphoribosyltransferase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenine phosphoribosyltransferase antibody
    • AMP antibody
    • AMP diphosphorylase antibody
    • AMP pyrophosphorylase antibody
    • APRT antibody
    • APT_HUMAN antibody
    • DKFZp686D13177 antibody
    • MGC125856 antibody
    • MGC125857 antibody
    • MGC129961 antibody
    • Transphosphoribosidase antibody
    see all

Images

  • Anti-APRT antibody (ab72782) at 1/500 dilution + human liver lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 20 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-APRT antibody (ab72782) at 1/500 dilution

    Lane 1 : APRT transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 20 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Naesens L  et al. Role of human hypoxanthine guanine phosphoribosyltransferase in activation of the antiviral agent T-705 (favipiravir). Mol Pharmacol 84:615-29 (2013). Read more (PubMed: 23907213) »
  • Kim JS  et al. Chloride intracellular channel 1 identified using proteomic analysis plays an important role in the radiosensitivity of HEp-2 cells via reactive oxygen species production. Proteomics 10:2589-2604 (2010). WB ; Human . Read more (PubMed: 20461716) »

See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Hamster Cell lysate - whole cell (Chinese hamster ovary cells)
Gel Running Conditions
Reduced Denaturing (12% resolving gel / 4% stacking gel)
Loading amount
200 µg
Specification
Chinese hamster ovary cells
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

Submitted Oct 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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