Overview

  • Product name
  • Description
    Rabbit polyclonal to APRT
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the C-terminal region of Human APRT conjugated to KLH (NP_000476).

  • Positive control
    • 293 cell lysates transiently transfected with the APRT gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab91428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 20 kDa.
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
  • Pathway
    Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
  • Involvement in disease
    Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:102600]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.
  • Sequence similarities
    Belongs to the purine/pyrimidine phosphoribosyltransferase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenine phosphoribosyltransferase antibody
    • AMP antibody
    • AMP diphosphorylase antibody
    • AMP pyrophosphorylase antibody
    • APRT antibody
    • APT_HUMAN antibody
    • DKFZp686D13177 antibody
    • MGC125856 antibody
    • MGC125857 antibody
    • MGC129961 antibody
    • Transphosphoribosidase antibody
    see all

Images

  • All lanes : Anti-APRT antibody (ab91428) at 1/100 dilution

    Lane 1 : 293 cell lysates (nontransfected)
    Lane 2 : 293 cell lysates transiently transfected with the APRT gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 20 kDa
  • ICC/IF image of ab91428 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91428, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab91428 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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