Overview

  • Product name
  • Description
    Rabbit polyclonal to APRT
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 104 of APRT.

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells; IHC-P: DLD-1 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab96025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 20 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
  • Pathway
    Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
  • Involvement in disease
    Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:102600]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.
  • Sequence similarities
    Belongs to the purine/pyrimidine phosphoribosyltransferase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenine phosphoribosyltransferase antibody
    • AMP antibody
    • AMP diphosphorylase antibody
    • AMP pyrophosphorylase antibody
    • APRT antibody
    • APT_HUMAN antibody
    • DKFZp686D13177 antibody
    • MGC125856 antibody
    • MGC125857 antibody
    • MGC129961 antibody
    • Transphosphoribosidase antibody
    see all

Anti-APRT antibody images

  • Anti-APRT antibody (ab96025) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 20 kDa
  • Top panel: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96025 at 1/200 dilution.
    Bottom panel: merged with DNA probe.
  • ab96025, at 1/500 dilution, staining APRT in formalin-fixed, paraffin-embedded DLD-1 xenograft by immunohistochemistry.

References for Anti-APRT antibody (ab96025)

ab96025 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Hamster Cell lysate - whole cell (Chinese Hamster Ovary (CHO) cells)
Gel Running Conditions
Reduced Denaturing (12 % resolving gel // 4% stacking gel)
Loading amount
100 µg
Specification
Chinese Hamster Ovary (CHO) cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Oct 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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