Overview

  • Product name
  • Description
    Rabbit polyclonal to APRT
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 104 of APRT.

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells; IHC-P: DLD-1 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab96025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 20 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
  • Pathway
    Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
  • Involvement in disease
    Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:102600]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.
  • Sequence similarities
    Belongs to the purine/pyrimidine phosphoribosyltransferase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenine phosphoribosyltransferase antibody
    • AMP antibody
    • AMP diphosphorylase antibody
    • AMP pyrophosphorylase antibody
    • APRT antibody
    • APT_HUMAN antibody
    • DKFZp686D13177 antibody
    • MGC125856 antibody
    • MGC125857 antibody
    • MGC129961 antibody
    • Transphosphoribosidase antibody
    see all

Anti-APRT antibody images

  • Anti-APRT antibody (ab96025) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 20 kDa
  • Top panel: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96025 at 1/200 dilution.
    Bottom panel: merged with DNA probe.
  • ab96025, at 1/500 dilution, staining APRT in formalin-fixed, paraffin-embedded DLD-1 xenograft by immunohistochemistry.

References for Anti-APRT antibody (ab96025)

ab96025 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab96025.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up