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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 20 kDa.
1/100 - 1/500.
1/100 - 1/200.
Function Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Pathway Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
Involvement in disease Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD) [MIM:102600]; also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones.
Sequence similarities Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Cellular localization Cytoplasm.
Information by UniProt
Adenine phosphoribosyltransferase antibody
AMP diphosphorylase antibody
Anti-APRT antibody images
Western blot - APRT antibody (ab96025)
Anti-APRT antibody (ab96025) at 1/1000 dilution + A431 whole cell lysate at 30 µg Predicted band size : 20 kDa
Immunocytochemistry/ Immunofluorescence - APRT antibody (ab96025)
Top panel: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96025 at 1/200 dilution. Bottom panel: merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - APRT antibody (ab96025)
ab96025, at 1/500 dilution, staining APRT in formalin-fixed, paraffin-embedded DLD-1 xenograft by immunohistochemistry.
References for Anti-APRT antibody (ab96025)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"