Anti-AQP11 antibody (ab122821)
Key features and details
- Rabbit polyclonal to AQP11
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AQP11 antibody -
Description
Rabbit polyclonal to AQP11 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: TLVGTSSNPC GVMMQMMLGG MSPETGAVRL LAQ, corresponding to amino acids 92-124 of Human AQP11.
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Positive control
- Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Function
Aquaporins facilitate the transport of water and small neutral solutes across cell membranes. -
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family. AQP11/AQP12 subfamily. -
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 282679 Human
- Omim: 609914 Human
- SwissProt: Q8NBQ7 Human
- Unigene: 503345 Human
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Alternative names
- AQP-11 antibody
- Aqp11 antibody
- AQP11_HUMAN antibody
- Aquaporin-11 antibody
Images
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab122821 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122821, at 1/20 dilution, staining AQP11 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab122821 has been referenced in 2 publications.
- Pellavio G et al. Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide Permeability. Int J Mol Sci 23:N/A (2022). PubMed: 36142747
- Guan Y et al. Effects of dexamethasone on C6 cell proliferation, migration and invasion through the upregulation of AQP1. Oncol Lett 15:7595-7602 (2018). PubMed: 29740485