The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 24376529
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/1000. Predicted molecular weight: 28 kDa.
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Belongs to the MIP/aquaporin (TC 1.A.8) family.
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
References for Anti-Aquaporin 1 antibody [1/A5F6] (ab11023)
This product has been referenced in:
Fontijn RD et al. Expression of Nitric Oxide-Transporting Aquaporin-1 Is Controlled by KLF2 and Marks Non-Activated Endothelium In Vivo. PLoS One10:e0145777 (2015).
Read more (PubMed: 26717516) »
Azouzi S et al. Energetic and molecular water permeation mechanisms of the human red blood cell urea transporter B. PLoS One8:e82338 (2013).
Read more (PubMed: 24376529) »