Overview

  • Product nameAnti-Aquaporin 1 antibody [7D11]
    See all Aquaporin 1 primary antibodies
  • Description
    Mouse monoclonal [7D11] to Aquaporin 1
  • Specificityab11025 recognises an epitope within an extracellular region of the water-specific channel aquaporin 1.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 198-208 of Rat Aquaporin 1.

  • Positive control
    • Kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified from tissue culture supernatant.
  • ClonalityMonoclonal
  • Clone number7D11
  • MyelomaSp2/0-Ag14
  • IsotypeIgG2b
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab11025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/500 - 1/1000.
WB 1/1000 - 1/2000. Predicted molecular weight: 32.28 kDa.
ELISA 1/1000 - 1/10000.

Target

  • FunctionForms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificityExpressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
  • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

References for Anti-Aquaporin 1 antibody [7D11] (ab11025)

This product has been referenced in:
  • Benfenati V  et al. An aquaporin-4/transient receptor potential vanilloid 4 (AQP4/TRPV4) complex is essential for cell-volume control in astrocytes. Proc Natl Acad Sci U S A 108:2563-8 (2011). WB ; Rat . Read more (PubMed: 21262839) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Vlady Pavlova

Verified customer

Submitted Aug 16 2006

I'm sorry to hear you are experiencing problems with ab11025. This antibody has not been tested in immunofluorescence and this may be the reason for the problem. I would recommend trying a biotin-ABC-DAB detection system which is more sensitive that ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"