The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/4000. Predicted molecular weight: 29 kDa. 0.5 µg/ml of ab125041 was sufficient for detection of
Aquaporin 1 in 10µg of Rat kidney tissue lysate by
colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
1/200 - 1/400.
FunctionForms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Tissue specificityExpressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).