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A 19 amino acid synthetic peptide within the carboxy terminal domain of rat Aquaporin 1 that is 100% homologous in mouse, rat, human and Bovine. This domain is predicted to be cytoplasmic.
Our Abpromise guarantee covers the use of ab15080 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 32 kDa.Can be blocked with Aquaporin 1 peptide (ab25381).|
|ELISA||1/10000 - 1/50000.|
ab15080 staining Aquaporin 1 in the intervertebral disc (nucleus pulposus) of the cow cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/200) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/200).
ab15080 at 1/500 dilution, staining Aquaporin 1 in sheep duodenum section by immunohistochemistry (Frozen sections). Sections were paraformaldehyde fixed and incubated with ab15080, for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit Ig, diluted 1/400 was used as the secondary antibody.