Overview

  • Product name
  • Description
    Rabbit polyclonal to Aquaporin 1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Sheep, Cow, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Aquaporin 1.

    (Peptide available as ab97414.)

  • Positive control
    • This antibody gave a positive signal in both HEK293 and Jurkat whole cell lysates as well as the following tissue lysates: Human pancreas; Human heart; Human skeletal muscle.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).

Target

  • Function
    Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificity
    Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
  • Sequence similarities
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 1 antibody
    • AQP CHIP antibody
    • AQP-1 antibody
    • AQP1 antibody
    • AQP1_HUMAN antibody
    • aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) antibody
    • aquaporin 1 (Colton blood group) antibody
    • Aquaporin CHIP antibody
    • Aquaporin-1 antibody
    • Aquaporin-CHIP antibody
    • Aquaporin1 antibody
    • Channel forming integral protein 28kDa antibody
    • Channel like integral membrane protein 28 kDa antibody
    • CHIP 28 antibody
    • CHIP28 antibody
    • CO antibody
    • Colton blood group antibody
    • Growth factor induced delayed early response protein antibody
    • MGC26324 antibody
    • Urine water channel antibody
    • Water channel protein CHIP 29 antibody
    • Water channel protein CHIP29 antibody
    • Water channel protein for red blood cells and kidney proximal tubule antibody
    see all

Anti-Aquaporin 1 antibody images

  • All lanes : Anti-Aquaporin 1 antibody (ab87845) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-Aquaporin 1 antibody (ab87845)

ab87845 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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