1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Is unsuitable for IP.
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Belongs to the MIP/aquaporin (TC 1.A.8) family.
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Aquaporin 1 with ab168387 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 1 antibody [EPR11588(B)] (ab168387)This image is courtesy of an Abreview submitted by Kirk McManus (1894904)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling Aquaporin 1 with ab168387 at a dilution of 1/200. Cells were fixed with Paraformaldehyde and permeabilised with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.
The antibody produces a strong, membrane-associated labelling pattern in both MeOH and PFA fixed samples.
Wu Z et al. RNAi-mediated silencing of AQP1 expression inhibited the proliferation, invasion and tumorigenesis of osteosarcoma cells. Cancer Biol Ther16:1332-40 (2015).
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